Department of Organic Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar 47416-95447, Iran.
Chem Biodivers. 2012 Dec;9(12):2732-41. doi: 10.1002/cbdv.201100389.
The essential-oil and fatty-acid composition of the aerial parts of Ficaria kochii (Ledeb.) Iranshahr & Rech.f. native to Iran, and the antioxidant activity of various extracts of this plant were examined. The study by GC-FID and GC/MS analysis of the essential oil resulted in the identification of 61 compounds, representing 86.01% of the total oil composition. Phytol (10.49%), farnesol (7.72%), methyl linoleate (5.57%), and α-farnesene (4.96%) were the main components. The fatty-acid composition of the aerial parts of F. kochii was also analyzed by GC/MS. The major components were palmitic acid (25.9%), linolenic acid (25.3%), and linoleic acid (17.5%). Polyunsaturated fatty acids (PUFAs) were found in higher amounts than saturated fatty acids. The possible antioxidant activity of various extracts (prepared by using solvents with different polarity) of the F. kochii aerial parts was evaluated by screening for their 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, Fe(III) -reducing power, total antioxidant activity, and inhibitory activity in the linoleic acid-peroxidation system. H(2) O proved to be the most efficient solvent for the extraction of antioxidants, as the H(2) O extract contained the highest amount of phenolic compounds (2.78±0.23 GAE/g dry matter) and also exhibited the strongest antioxidant capacity in all the assays used. The results of the present investigation demonstrated that the aerial parts of F. kochii can be used as natural and safe nutrition supplement in place of synthetic ones.
伊朗土生土长的 Ficaria kochii（Ledeb.）Iranshahr & Rech.f. 的地上部分的精油和脂肪酸组成以及该植物不同提取物的抗氧化活性进行了研究。通过 GC-FID 和 GC/MS 分析精油，鉴定出 61 种化合物，占总油成分的 86.01%。植物醇（10.49%）、法呢醇（7.72%）、亚油酸甲酯（5.57%）和α-法呢烯（4.96%）是主要成分。通过 GC/MS 还分析了 F. kochii 地上部分的脂肪酸组成。主要成分是棕榈酸（25.9%）、亚麻酸（25.3%）和亚油酸（17.5%）。多不饱和脂肪酸（PUFAs）的含量高于饱和脂肪酸。通过筛选 2,2-二苯基-1-苦基肼（DPPH）自由基清除活性、Fe(III)还原能力、总抗氧化活性和抑制亚油酸过氧化体系中的活性，评估了 F. kochii 地上部分不同提取物（用不同极性溶剂制备）的可能抗氧化活性。结果表明，H2O 是提取抗氧化剂最有效的溶剂，因为 H2O 提取物含有最高量的酚类化合物（2.78±0.23 GAE/g 干物质），并且在所有使用的测定中表现出最强的抗氧化能力。本研究结果表明，F. kochii 的地上部分可用作天然和安全的营养补充剂，以替代合成品。
