Guan Han-Liang, Qian Da-Wei, Duan Jin-Ao, Ren Hao, Qian Ye-Fei, Tang Yu-Ping, Liu Pei
Nanjing University of Chinese Medicine, Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing 210046, China.
Zhongguo Zhong Yao Za Zhi. 2013 Jul;38(13):2140-6.
To provide a scientific evidence for the initial primary processing method, an ultra-high performance liquid chromatography combined with a triple quadrupole electrospray tandem mass spectrometry (UPLC-MS/MS) was used to analyze the contents variation of catechins, flavonoids, flavonoid glycosides, biflavones, terpene lactones and phenolic acids during the process of drying in the sun, in the shade, and baked with 35, 45, 60, 80 degrees C, respectively. The results show that drying in the 80 degrees C is conducive to the accumulation of catechins, flavonoid glycosides, terpene lactones, better than the effects of other procedures. Therefore, the fast drying at 80 degrees C is beneficial for the retention of various types of active ingredient of Ginkgo biloba, and this method could be applied as a preferably dry processing.
为了给初始的初级加工方法提供科学依据，采用超高效液相色谱结合三重四极杆电喷雾串联质谱（UPLC-MS/MS）分别分析了银杏叶在晒、阴干以及在35、45、60、80℃烘烤过程中儿茶素、黄酮类、黄酮苷、双黄酮、萜类内酯和酚酸的含量变化。结果表明，80℃烘烤有利于儿茶素、黄酮苷、萜类内酯的积累，优于其他处理方式。因此，80℃快速干燥有利于银杏叶各类活性成分的保留，该方法可作为一种优选的干燥加工方式。
