Liu Chun-Fang, Tan Shu-Fang, Wang Dan-Hua, Zhang Yan-Qiong, Lin Na
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2013 Jul;38(13):2169-75.
To study the effect of Aconiti Radix Cocta and Pinelliae Rhizoma with different matching proportions and doses on their analgic, anti-inflammatory, phlegm eliminating and cough relieving efficacies in mice.
The two-factor, seven-level uniform design method was adopted to observe the effect of the oral administration with the combined decoction on the analgic, anti-inflammatory, phlegm eliminating and cough relieving efficacies, with frequency of body torsions induced by acetum, ear swelling degree induced by dimethylbenzene, secretion of phenol red in tracheas and frequency of coughs induced by aqueous ammonia as indexes. Significant matching proportions and doses were collected for verification.
(1) The effect on the frequency of body torsions and ear swelling degree. The combined decoction could effectively reduce the frequency of body torsions and ear swelling degree. According to a regression analysis, Aconiti Radix Cocta and Pinelliae Rhizoma had the antagonism, which was maximized at the ratio of 10: 1, and minimized at the ratio of less than or equal to 1: 1. The frequency of body torsions and ear swelling degree increased first and then decreased along with the rise in the total dose; and a higher proportion of Aconiti Radix Cocta resulted in a faster speed in decrease or increase. (2) The effect on the secretion of phenol red in tracheas and frequency of coughs. The combined decoction could effectively increase the secretion of phenol red in tracheas and decrease the frequency of coughs. According to a regression analysis, Pinelliae Rhizoma and Aconiti Radix Cocta had the synergistic effect in the secretion of phenol red in tracheas, which was maximized with a total dose of more than 5 g x kg(-1) and a ratio of 1: 1.
The compatible application of Pinelliae Rhizoma and Aconiti Radix Cocta can decrease the analgesic and anti-inflammatory effects of Aconiti Radix Cocta and promote the cough-relieving effect of Pinelliae Rhizoma, which vary according to different matching ratio and dose. This study provides experimental basis for indepth studies on the combined effect of Aconiti Radix Cocta and Pinelliae Rhizoma--two of eighteen incompatible pairs.
研究不同配比及剂量的制川乌与半夏对小鼠的镇痛、抗炎、祛痰、止咳作用。
采用二因素七水平均匀设计法，以醋酸致小鼠扭体次数、二甲苯致小鼠耳肿胀度、气管酚红排泌量及氨水致小鼠咳嗽次数为指标，观察合煎剂灌胃给药后的镇痛、抗炎、祛痰、止咳作用，并收集有显著意义的配比及剂量进行验证。
（1）对小鼠扭体次数及耳肿胀度的影响。合煎剂能有效减少小鼠扭体次数及耳肿胀度。回归分析显示，制川乌与半夏有拮抗作用，在10∶1时拮抗作用最大，小于或等于1∶1时最小。小鼠扭体次数及耳肿胀度随总剂量增加先升高后降低，制川乌比例越高升降速度越快。（2）对气管酚红排泌量及咳嗽次数的影响。合煎剂能有效增加气管酚红排泌量，减少咳嗽次数。回归分析显示，半夏与制川乌在气管酚红排泌量方面有协同作用，总剂量大于5 g·kg-1、比例为1∶1时协同作用最大。
半夏与制川乌配伍应用可降低制川乌的镇痛、抗炎作用，增强半夏的止咳作用，且作用随配比及剂量不同而变化。本研究为十八反中制川乌与半夏配伍效应的深入研究提供了实验依据。
