[荷叶碱对肝星状细胞中Ⅰ型胶原、基质金属蛋白酶抑制因子-1及基质金属蛋白酶-2的影响]

[Effect of neferine on hepatic stellate cells in collagen-I, TIMP-1 and MMP-2].

作者信息

Li Xian-Mei, Dong Lei, Shi Hai-Tao, Gao Tian-Jiao, Jia Miao

机构信息

Department of Gastroenterology, Second Hospital Affiliated to College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2013 Jul;38(13):2206-9.

PMID:24079255

Abstract

OBJECTIVE

To observe the effect of neferine on Collagen-I, TIMP-1 and MMP-2 expressions and protein secretion of hepatic stellate cells.

METHOD

The hepatic stellate cell line HSC-T6 was cultured in vitro, and then randomly divided into 5 groups: the control group, the platelet-derived growth factor (PDGF) group and PDGF + neferine (2, 6, 10 micromol x L(-1)) groups. All of the groups were cultured for 48 h, and their cells were collected to extract mRNA and detect Collagen-I, TIMP-1 and MMP-2 expressions with RT-PCR. Their cell supernatants were also collected to determine the protein content of three factors with ELISA.

RESULT

Compared with the control group, PDGF could remarkably increase the Collagen-I, TIMP-1 and MMP-2 expressions and protein secretion of hepatic stellate cells. Compared with the PDGF group, PDGF + neferine (6, 10 micromol x L(-1)) groups showed a notable decrease in the Collagen-I and mRNA expression and protein secretion along with the increase in the concentration, whereas the PDGF + neferine (2 micromol x L(-1)) group showed no significant change in the Collagen-I and mRNA expression and protein secretion. Compared with the PDGF group, three PDGF + neferine groups showed no notable change in MMP-2 expression and protein secretion.

CONCLUSION

Neferine can inhibit the Collagen-I, TIMP-1 and mRNA protein expression and protein secretion of PDGF-induced HSCs along with the increase in the concentration, but with not remarkable effect on the MMP-2 expression and secretion.

摘要

目的

观察甲基莲心碱对肝星状细胞Ⅰ型胶原蛋白（Collagen-I）、基质金属蛋白酶组织抑制因子-1（TIMP-1）和基质金属蛋白酶-2（MMP-2）表达及蛋白分泌的影响。

方法

体外培养肝星状细胞系HSC-T6，然后随机分为5组：对照组、血小板衍生生长因子（PDGF）组和PDGF + 甲基莲心碱（2、6、10 μmol·L⁻¹）组。所有组均培养48 h，收集细胞提取mRNA，采用逆转录-聚合酶链反应（RT-PCR）检测Collagen-I、TIMP-1和MMP-2的表达。同时收集细胞上清液，采用酶联免疫吸附测定（ELISA）法测定三种因子的蛋白含量。

结果

与对照组相比，PDGF可显著增加肝星状细胞Collagen-I、TIMP-1和MMP-2的表达及蛋白分泌。与PDGF组相比，PDGF + 甲基莲心碱（6、10 μmol·L⁻¹）组随着浓度增加，Collagen-I及mRNA表达和蛋白分泌显著降低，而PDGF + 甲基莲心碱（2 μmol·L⁻¹）组Collagen-I及mRNA表达和蛋白分泌无明显变化。与PDGF组相比，三个PDGF + 甲基莲心碱组MMP-2表达和蛋白分泌无明显变化。