Tang Longhua, Chun Ik Su, Wang Zidong, Li Jinghong, Li Xiuling, Lu Yi
Department of Chemistry, Beijing Key Laboratory for Analytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University , Beijing, China 100084.
Anal Chem. 2013 Oct 15;85(20):9522-7. doi: 10.1021/ac401169c. Epub 2013 Sep 30.
Efficient near-infrared detection of specific DNA with single nucleotide polymorphism selectivity is important for diagnostics and biomedical research. Herein, we report the use of gallium arsenide (GaAs) as a sensing platform for probing DNA immobilization and targeting DNA hybridization, resulting in ∼8-fold enhanced GaAs photoluminescence (PL) at ∼875 nm. The new signal amplification strategy, further coupled with the plasmonic effect of Au nanoparticles, is capable of detecting DNA molecules with a detection limit of 0.8 pM and selectivity against single base mismatches. Such an ultrasensitive near-infrared sensor can find a wide range of biochemical and biomedical applications.
对具有单核苷酸多态性选择性的特定DNA进行高效近红外检测，对诊断和生物医学研究具有重要意义。在此，我们报道了使用砷化镓（GaAs）作为传感平台来探测DNA固定化和靶向DNA杂交，从而在约875nm处使GaAs光致发光（PL）增强约8倍。这种新的信号放大策略，再加上金纳米颗粒的等离子体效应，能够检测DNA分子，检测限为0.8 pM，并且对单碱基错配具有选择性。这样一种超灵敏的近红外传感器可在广泛的生化和生物医学应用中找到用武之地。
