Marquez Daniela T, Chawich Juliana, Hassen Walid M, Moumanis Khalid, DeRosa Maria C, Dubowski Jan J
Interdisciplinary Institute for Technological Innovation (3IT), CNRS UMI-3463, Université de Sherbrooke, 3000, Boulevard de l'Université, Sherbrooke, Québec J1K 0A5, Canada.
Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada.
ACS Omega. 2021 Mar 12;6(11):7286-7295. doi: 10.1021/acsomega.0c04954. eCollection 2021 Mar 23.
Despite evidence showing that polymer brushes (PBs) are a powerful tool used in biosensing for minimizing nonspecific interactions, allowing for optimization of biosensing performance, and the fact that GaAs semiconductors have proven to have a remarkable potential for sensitive biomolecule detection, the combination of these two robust components has never been considered nor evaluated as a platform for biosensing applications. This work reports different methodologies to prepare and tune PBs on the GaAs interface (PB-GaAs) and their potential as useful platforms for antibody grafting, with the ultimate goal of demonstrating the innovative and attractive character of the PB-GaAs interfaces in the enhanced capture of antibodies and control of nonspecific interactions. Three different functionalization approaches were explored, one "grafting-to" and two "grafting-from," in which atom transfer radical polymerization (ATRP) was performed, followed by their corresponding characterizations. Demonstration of the compatibility of () and () antibodies with the PB-GaAs platform compared to the results obtained with conventional biosensing architectures developed for GaAs indicates the attractive potential for operation of a sensitive biosensor. Furthermore, these results showed that by carefully choosing the nature and preparation methodology of a PB-GaAs interface, it is possible to effectively tune the affinity of PB-GaAs-based sensors toward and Lp antibodies ultimately demonstrating the superior specificity of the developed biosensing platform.
尽管有证据表明聚合物刷（PBs）是生物传感中用于最小化非特异性相互作用、优化生物传感性能的有力工具，且砷化镓（GaAs）半导体已被证明在灵敏生物分子检测方面具有显著潜力，但这两种强大组件的组合从未被视为或评估为生物传感应用的平台。这项工作报道了在GaAs界面上制备和调节聚合物刷（PB-GaAs）的不同方法及其作为抗体接枝有用平台的潜力，最终目标是证明PB-GaAs界面在增强抗体捕获和控制非特异性相互作用方面的创新性和吸引力。探索了三种不同的功能化方法，一种是“接枝到”，两种是“从接枝”，其中进行了原子转移自由基聚合（ATRP），随后进行了相应的表征。与为GaAs开发的传统生物传感架构所获得的结果相比，（）抗体和（）抗体与PB-GaAs平台的兼容性证明了灵敏生物传感器运行的诱人潜力。此外，这些结果表明，通过仔细选择PB-GaAs界面的性质和制备方法，可以有效调节基于PB-GaAs的传感器对和Lp抗体的亲和力，最终证明所开发生物传感平台的卓越特异性。
