基于 C 点和芬顿反应的用于检测胆碱和乙酰胆碱的环保、简单、灵敏的荧光生物传感器。
An eco-friendly, simple, and sensitive fluorescence biosensor for the detection of choline and acetylcholine based on C-dots and the Fenton reaction.
机构信息
Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, No. 29, Zhongguancun East Road, Haidian District, Beijing 100190, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
出版信息
Biosens Bioelectron. 2014 Feb 15;52:304-9. doi: 10.1016/j.bios.2013.09.006. Epub 2013 Sep 14.
A simple and novel method is proposed for the preparation of Carbon dots (C-dots) with excellent properties. We firstly demonstrated that the fluorescence of C-dots decreased apparently in the presence of H2O2 and Fe(2+). Based on the this finding, C-dots are successfully adopted as probes for the detection of H2O2. After the experimental conditions are optimized, the limit of detection (LOD) for H2O2 is found to be 0.1 μM. Furthermore, we established an eco-friendly, simple and sensitive biosensor for the detection of choline and acetylcholine (ACh) based on the detection of H2O2 using C-dots as probes. The detection limit for choline is 0.1 μM and the linear range is 0.1-40 μM. The detection limit for ACh is found to be 0.5 μM and the linear range is 0.5-60 μM. The excellent performance of the proposed biosensor shows that this method possesses the potential for practical application.
提出了一种简单新颖的方法来制备具有优异性能的碳点（C-dots）。我们首先证明了在存在 H2O2 和 Fe(2+)的情况下，C-dots 的荧光明显降低。基于这一发现，C-dots 成功地被用作检测 H2O2 的探针。在优化实验条件后，发现 H2O2 的检测限（LOD）为 0.1 μM。此外，我们建立了一种基于 C-dots 作为探针检测 H2O2 的用于检测胆碱和乙酰胆碱（ACh）的环保、简单和灵敏的生物传感器。检测胆碱的检测限为 0.1 μM，线性范围为 0.1-40 μM。检测 ACh 的检测限为 0.5 μM，线性范围为 0.5-60 μM。所提出的生物传感器具有优异的性能，表明该方法具有实际应用的潜力。
Zotero 插件