Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
Biosens Bioelectron. 2011 Oct 15;28(1):50-5. doi: 10.1016/j.bios.2011.06.041. Epub 2011 Jul 6.
In this paper, we have constructed a simple, rapid and sensitive biosensor for detection of choline and acetylcholine (ACh) based on the hydrogen peroxide (H(2)O(2))-sensitive quantum dots (QDs). The detection limit for choline was 0.1 μM and the linear range was 0.1-0.9 μM and 5-150 μM, respectively. The detection limit for ACh was found to be 10 μM and the linear range was 10-5000 μM. The wide linear ranges were shown to be suitable for routine analyses of choline and ACh. Possible mechanism of the fluorescence of QDs quenched by H(2)O(2) was an electron transfer (ET) process. The experimental conditions of biosensors were optimized, and anti-interference ability was also presented. We also detected the choline in milk samples and the linear range was 5-150 μM. The detection linear range of ACh in serum was 10-140 μM. Most importantly, the recovery of choline in milk and ACh in serum samples were both close to 99%. The excellent performance of this biosensor showed that the method can be used in practice detection of choline and ACh.
在本文中，我们构建了一种基于对过氧化氢（H₂O₂）敏感的量子点（QDs）的简单、快速和灵敏的胆碱和乙酰胆碱（ACh）生物传感器。胆碱的检测限为 0.1 μM，线性范围分别为 0.1-0.9 μM 和 5-150 μM。ACh 的检测限为 10 μM，线性范围为 10-5000 μM。宽线性范围适用于胆碱和 ACh 的常规分析。QDs 被 H₂O₂ 猝灭的荧光可能的机制是电子转移（ET）过程。优化了生物传感器的实验条件，并提出了抗干扰能力。我们还检测了牛奶样品中的胆碱，线性范围为 5-150 μM。血清中 ACh 的检测线性范围为 10-140 μM。最重要的是，牛奶中胆碱和血清中 ACh 的回收率均接近 99%。该生物传感器的优异性能表明该方法可用于实际检测胆碱和 ACh。
