Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600113, India.
Marine Bioprocess Research Center, Department of Chemistry, Pukyong National University, Busan 608-737, South Korea.
Clin Nutr. 2014 Aug;33(4):718-26. doi: 10.1016/j.clnu.2013.08.011. Epub 2013 Sep 3.
BACKGROUND & AIM: Prostate cancer is one of the frequently diagnosed cancers in men. Increased Growth factor IGF-1/IGF-1R axis activation mediated by both PI3K/Akt or RAF/MEK/ERK system and AR expression remains important in the development and progression of prostate cancer. Targeting such system by dietary agents quercetin in vivo model could aid its application in both treatment as well as prevention of prostate cancer.
In our study the rats were divided into four groups; Group I: control (propylene glycol-vehicle), Group II: cancer-induced (MNU and Testosterone treated) rats, Group III: cancer-induced + Quercetin (200 mg/kg body wt/orally) and Group IV: Quercetin (200 mg/kg body wt) thrice a week. After the treatment period rats were sacrificed and the ventral and dorsolateral prostate lobes were dissected.
Antioxidant enzymes and apoptotic proteins were significantly decreased in cancer-induced animal and upon quercetin supplement its level was increased. The IGFIR, AKT, AR, cell proliferative and anti-apoptotic proteins were increased in cancer-induced group whereas supplement of quercetin decreased its expression.
Quercetin down regulates the cell survival, proliferative and anti-apoptotic proteins thereby prevents prostate cancer, by acting as a chemopreventive agent in preclinical model.
前列腺癌是男性中常见的癌症之一。由 PI3K/Akt 或 RAF/MEK/ERK 系统和 AR 表达介导的生长因子 IGF-1/IGF-1R 轴的激活增加在前列腺癌的发展和进展中仍然很重要。通过饮食因子槲皮素在体内模型中靶向该系统可以帮助其在前列腺癌的治疗和预防中应用。
在我们的研究中，大鼠被分为四组；第 I 组：对照组（丙二醇-载体），第 II 组：癌症诱导组（MNU 和睾酮处理），第 III 组：癌症诱导+槲皮素（200mg/kg 体重/口服），第 IV 组：槲皮素（200mg/kg 体重）每周 3 次。治疗期结束后，处死大鼠，解剖腹侧和背外侧前列腺叶。
抗氧化酶和凋亡蛋白在癌症诱导的动物中显著降低，而槲皮素补充后其水平增加。IGFIR、AKT、AR、细胞增殖和抗凋亡蛋白在癌症诱导组中增加，而槲皮素的补充则降低了其表达。
槲皮素通过作为临床前模型中的化学预防剂，下调细胞存活、增殖和抗凋亡蛋白，从而预防前列腺癌。
