Mei Yu-xia, Chen Hong-xia, Zhang Jun, Zhang Xue-dan, Liang Yun-xiang
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Int J Biol Macromol. 2013 Nov;62:330-5. doi: 10.1016/j.ijbiomac.2013.09.038. Epub 2013 Sep 27.
Chitooligosaccharides (COS) display a variety of important biological activities, including antimicrobial, antitumor, anti-inflammatory, and immunoenhancing. In the present study, a COS sample (degree of polymerization 4-11, analyzed by FTIR and MALDI-TOF-MS) prepared by hydrolysis with a recombinant chitosanase was orally administered to mice for evaluation of its effect on cyclophosphamide (Cy)-induced immunosuppression. Thymus and spleen indices, delayed-type hypersensitivity (DTH) reaction, macrophage phagocytosis, and certain enzyme activities were significantly higher in mice treated with COS+Cy than in mice treated with Cy alone. ELISA experiments showed that COS treatment enhanced production of the cytokines IL-2, IL-12, and IFN-γ but decreased production of IL-10 in sera of Cy-treated mice. The well-defined COS product studied displayed strong immunoenhancing activity and a protective effect against Cy-induced immunosuppression. COS-derived products should be further investigated for possible immunostimulatory applications in the food and pharmaceutical industries.
壳寡糖（COS）具有多种重要的生物活性，包括抗菌、抗肿瘤、抗炎和免疫增强作用。在本研究中，将通过重组壳聚糖酶水解制备的COS样品（聚合度4 - 11，通过傅里叶变换红外光谱（FTIR）和基质辅助激光解吸电离飞行时间质谱（MALDI - TOF - MS）分析）口服给予小鼠，以评估其对环磷酰胺（Cy）诱导的免疫抑制的影响。与仅用Cy处理的小鼠相比，用COS + Cy处理的小鼠的胸腺和脾脏指数、迟发型超敏反应（DTH）、巨噬细胞吞噬作用以及某些酶活性显著更高。酶联免疫吸附测定（ELISA）实验表明，COS处理可增强Cy处理小鼠血清中细胞因子白细胞介素 - 2（IL - 2）、白细胞介素 - 12（IL - 12）和干扰素 - γ（IFN - γ）的产生，但降低白细胞介素 - 10（IL - 10）的产生。所研究的明确的COS产品显示出强大的免疫增强活性以及对Cy诱导的免疫抑制的保护作用。COS衍生产品应进一步研究其在食品和制药行业中可能的免疫刺激应用。
