*Department of Otolaryngology Head and Neck Surgery, Universidad Nacional de Colombia; †Division of Otology and Neurotology, Fundación Universitaria Ciencias de la Salud; ‡Department of Hand and Peripheral Nerve Surgery, Hospital Simón Bolívar, Universidad El Bosque, Bogota; and §Faculty of Sciences, Department of Biology, Universidad Nacional de Colombia, Cundinamarca, Colombia.
Otol Neurotol. 2014 Feb;35(2):e64-8. doi: 10.1097/MAO.0b013e31829e1680.
To compare axonal regeneration in an animal model after the repair of facial nerve defects with pre-degenerated (PD) and non-PD (NPD) great auricular nerve grafts.
The buccal branch of the facial nerve was completely sectioned in 18 New Zealand rabbits, which were randomized to 3 treatment groups: PD great auricular nerve graft repair (PD group), NPD great auricular nerve graft repair (NPD group), and immediate end-to-end repair (control group). Axonal regeneration was examined using optical microscopy to assess the following variables: total number of myelinated axons and regenerating sprouts (MARS) that crossed the distal anastomosis, numbers of intra and extrafascicular MARS, and area of myelinated axons.
The total number of myelinated axons (p = 0.008) and intrafascicular axons (p = 0.02) that crossed the distal anastomosis significantly differed between the NPD and control group. No significant differences were observed between the PD and control group or between the PD and NPD groups.
Nerve repair with PD grafts could be an alternative treatment in the management of injuries resulting in facial nerve gaps.
比较修复面神经缺损时，预先退化（PD）和非 PD（NPD）耳大神经移植物后的轴突再生情况。
18 只新西兰兔的面神经颊支被完全切断，随机分为 3 组治疗：PD 耳大神经移植物修复（PD 组）、NPD 耳大神经移植物修复（NPD 组）和即时端端修复（对照组）。用光镜评估以下变量来检查轴突再生：穿过远端吻合的有髓轴突和再生芽（MARS）总数、束内和束外 MARS 数量以及有髓轴突面积。
穿过远端吻合的有髓轴突总数（p=0.008）和束内轴突数（p=0.02）在 NPD 组和对照组之间有显著差异。PD 组与对照组之间或 PD 组与 NPD 组之间无显著差异。
PD 移植物神经修复可能是面神经间隙损伤治疗的一种替代方法。
