Corbellini Silvia, Piccinelli Giorgio, De Francesco Maria Antonia, Ravizzola Giuseppe, Bonfanti Carlo
Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia, P. le Spedali Civili, 1, 25123, Brescia, Italy.
Folia Microbiol (Praha). 2014 Mar;59(2):173-9. doi: 10.1007/s12223-013-0281-3. Epub 2013 Oct 1.
The purpose of this study is to analyze isolates of Clostridium difficile from patients with nosocomial acquired infection in respect to their molecular type and antimicrobial susceptibility. Fifty-nine randomly selected clinical isolates were characterized. Molecular typing was performed by rep-PCR (DiversiLab). Isolates were tested by disk diffusion towards 11 different antibiotics. All isolates were susceptible to metronidazole and vancomycin. Fifty five (93 %) isolates were resistant to erythromycin and fifty six (95 %) exhibited resistance to both clindamycin and moxifloxacin. Twenty rep-PCR types were identified, but most clinical isolates formed four major rep-PCR clusters (A1 24/59, 40 %; A2 20/59, 33 %; A3 5/59, 8 %; A4 3/59, 5 %). These results show high genetic variability, which demonstrate clearly the complexity of the strains of C. difficile and also show an increasing rate of resistance to fluoroquinolones in our region emphasizing the importance of implementing surveillance programs in order to prevent further spread of resistance in C. difficile.
本研究的目的是分析医院获得性感染患者艰难梭菌分离株的分子类型和抗菌药敏性。对59株随机选择的临床分离株进行了特征分析。采用rep-PCR（DiversiLab）进行分子分型。通过纸片扩散法对分离株进行11种不同抗生素的药敏试验。所有分离株对甲硝唑和万古霉素敏感。55株（93%）分离株对红霉素耐药，56株（95%）对克林霉素和莫西沙星均耐药。鉴定出20种rep-PCR类型，但大多数临床分离株形成了四个主要的rep-PCR簇（A1 24/59，40%；A2 20/59，33%；A3 5/59，8%；A4 3/59，5%）。这些结果显示出高度的遗传变异性，清楚地表明了艰难梭菌菌株的复杂性，也显示出我们地区对氟喹诺酮类药物的耐药率在上升，强调了实施监测计划以防止艰难梭菌耐药性进一步传播的重要性。
