对用于检测泌乳奶牛新孢子虫病和白血病的牛奶和血清样本进行酶联免疫吸附测定的评估。
Evaluation of enzyme-linked immunosorbent assays performed on milk and serum samples for detection of neosporosis and leukosis in lactating dairy cows.
作者信息
Walsh Robert B, Kelton David F, Hietala Sharon K, Duffield Todd F
机构信息
Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1 (Walsh, Kelton, Duffield); The California Animal Health and Food Safety Laboratory System, University of California, Davis Campus, PO Box 1770, Davis, California 95617, USA (Hietala).
出版信息
Can Vet J. 2013 Apr;54(4):347-52.
Serum and milk samples from 1229 cows on 22 Ontario dairy farms were individually tested for antibodies specific for bovine leukosis virus (BLV) and Neospora caninum by enzyme-linked immunosorbent assay (ELISA). Antibodies against BLV were present in 361 serum samples (29.4%) and 369 milk samples (30.0%). Comparing the 2 tests, agreement was almost perfect (k = 0.86; 95% CI = 0.83 to 0.90) and the proportions of samples positive were not significantly different (P = 0.56). Both tests identified the same 3 herds free of bovine leukosis virus. Antibodies against N. caninum were detected in 138 serum samples (11.2%), and 111 milk samples (9.0%). Agreement between the 2 tests was moderate (k = 0.52; 95% CI = 0.43 to 0.59). Four herds were free of neosporosis by the serum test, while 10 herds were negative by the milk test. The ELISA on milk samples facilitates sample collection to classify herds free of BLV; the milk N. caninum ELISA was less reliable in predicting herd-level infection.
通过酶联免疫吸附测定（ELISA）对安大略省22个奶牛场的1229头奶牛的血清和牛奶样本分别进行检测，以检测针对牛白血病病毒（BLV）和犬新孢子虫的特异性抗体。361份血清样本（29.4%）和369份牛奶样本（30.0%）中存在抗BLV抗体。比较这两种检测方法，一致性几乎完美（k = 0.86；95%置信区间 = 0.83至0.90），阳性样本比例无显著差异（P = 0.56）。两种检测方法均鉴定出相同的3个无牛白血病病毒的牛群。在138份血清样本（11.2%）和111份牛奶样本（9.0%）中检测到抗犬新孢子虫抗体。两种检测方法之间的一致性为中等（k = 0.52；95%置信区间 = 0.43至0.59）。血清检测显示有4个牛群无新孢子虫病，而牛奶检测显示有10个牛群为阴性。牛奶样本的ELISA有助于收集样本以对无BLV的牛群进行分类；牛奶中犬新孢子虫ELISA在预测牛群水平感染方面不太可靠。
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