Dubin D T, HsuChen C C
Plasmid. 1985 Mar;13(2):139-44. doi: 10.1016/0147-619x(85)90066-6.
3' Terminal sequences of mosquito mitochondrial 16 S ribosomal RNA, which is post-transcriptionally polyadenylated, have been examined with the aim of determining the degree of homogeneity of transcribed moieties. 3' End-labeled samples were subjected to oligonucleotide fingerprint analysis and to ladder gel analysis after primary and secondary nuclease digestion; and products of reverse transcriptase reactions were characterized using 16 S RNA as template and selected oligodeoxynucleotides as primers. The results indicated a remarkable degree of homogeneity compared to homologous mammalian mitochondrial systems, and suggested differences in modes of expression of insect, versus mammalian, mitochondrial genomes.
蚊子线粒体16S核糖体RNA的3'末端序列在转录后会进行多聚腺苷酸化，为了确定转录部分的同源程度，我们对其进行了研究。对3'末端标记的样品进行了寡核苷酸指纹分析，并在一级和二级核酸酶消化后进行了阶梯凝胶分析；以16S RNA为模板，选择寡聚脱氧核苷酸作为引物，对逆转录酶反应产物进行了表征。结果表明，与同源的哺乳动物线粒体系统相比，其同源程度非常高，这表明昆虫线粒体基因组与哺乳动物线粒体基因组的表达模式存在差异。
