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编码酵母线粒体大核糖体RNA的完整DNA序列。

Complete DNA sequence coding for the large ribosomal RNA of yeast mitochondria.

作者信息

Sor F, Fukuhara H

出版信息

Nucleic Acids Res. 1983 Jan 25;11(2):339-48. doi: 10.1093/nar/11.2.339.

DOI:10.1093/nar/11.2.339
PMID:6298722
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC325718/
Abstract

The mitochondrial gene coding for the large ribosomal RNA (21S) has been isolated from a rho- clone of Saccharomyces cerevisiae. A DNA segment of about 5500 base pairs has been sequenced which included the totality of the sequence coding for the mature ribosomal RNA and the intron. The mature RNA sequence corresponds to a length of 3273 nucleotides. Despite the very low guanine-cytosine content (20.5%), many stretches of sequence are homologous to the corresponding Escherichia coli 23S ribosomal RNA. The sequence can be folded into a secondary structure according to the general models for prokaryotic and eukaryotic large ribosomal RNAs. Like the E.coli gene, the mitochondrial gene contains the sequences that look like the eukaryotic 5.8S and the chloroplastic 4.5S ribosomal RNAs. The 5' and 3' end regions show a complementarity over fourteen nucleotides.

摘要

编码大核糖体RNA（21S）的线粒体基因已从酿酒酵母的一个ρ-克隆中分离出来。已对一段约5500个碱基对的DNA片段进行了测序，其中包括编码成熟核糖体RNA和内含子的全部序列。成熟RNA序列的长度为3273个核苷酸。尽管鸟嘌呤-胞嘧啶含量非常低（20.5%），但许多序列片段与相应的大肠杆菌23S核糖体RNA同源。根据原核生物和真核生物大核糖体RNA的一般模型，该序列可折叠成二级结构。与大肠杆菌基因一样，线粒体基因包含类似于真核生物5.8S和叶绿体4.5S核糖体RNA的序列。5'和3'末端区域在14个核苷酸上显示出互补性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7d/325718/9f8672bc33c0/nar00347-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7d/325718/9f8672bc33c0/nar00347-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7d/325718/9f8672bc33c0/nar00347-0115-a.jpg

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1
Complete DNA sequence coding for the large ribosomal RNA of yeast mitochondria.编码酵母线粒体大核糖体RNA的完整DNA序列。
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2
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引用本文的文献

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Curr Genet. 1984 Feb;8(2):121-6. doi: 10.1007/BF00420230.
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本文引用的文献

1
Secondary structure model for 23S ribosomal RNA.23S核糖体RNA的二级结构模型
Nucleic Acids Res. 1981 Nov 25;9(22):6167-89. doi: 10.1093/nar/9.22.6167.
2
Secondary structure of the large subunit ribosomal RNA from Escherichia coli, Zea mays chloroplast, and human and mouse mitochondrial ribosomes.来自大肠杆菌、玉米叶绿体以及人类和小鼠线粒体核糖体的大亚基核糖体RNA的二级结构。
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Splice point sequence and transcripts of the intervening sequence in the mitochondrial 21S ribosomal RNA gene of yeast.
海绵动物的线粒体基因组为重复发夹形成元件 (RHE) 的多次入侵提供了证据。
BMC Genomics. 2009 Dec 9;10:591. doi: 10.1186/1471-2164-10-591.
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Oxidative DNA damage causes mitochondrial genomic instability in Saccharomyces cerevisiae.氧化性DNA损伤导致酿酒酵母中的线粒体基因组不稳定。
Mol Cell Biol. 2005 Jun;25(12):5196-204. doi: 10.1128/MCB.25.12.5196-5204.2005.
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Uracil-DNA glycosylase-deficient yeast exhibit a mitochondrial mutator phenotype.尿嘧啶-DNA糖基化酶缺陷型酵母表现出线粒体突变体表型。
Nucleic Acids Res. 2001 Dec 15;29(24):4935-40. doi: 10.1093/nar/29.24.4935.
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Inactivation of Saccharomyces cerevisiae OGG1 DNA repair gene leads to an increased frequency of mitochondrial mutants.酿酒酵母OGG1 DNA修复基因的失活导致线粒体突变体频率增加。
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10
Patterns of mitochondrial sorting in yeast zygotes.酵母合子中线粒体分选模式。
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