Groenewegen G, Buurman W A, van der Linden C J, Jeunhomme G M, Kootstra G
Transplantation. 1985 Jun;39(6):657-60. doi: 10.1097/00007890-198506000-00016.
Knowledge of the antigens recognized during allograft rejection is still incomplete. Cloned cytotoxic T lymphocytes were used to study the distribution of target determinants in the dog. CTL clones were obtained with a limiting dilution technique from effector cells generated in mixed lymphocyte culture. The clones have been tested for cell-mediated cytotoxicity against PHA-stimulated lymphoblasts, monocytes, and arterial and venous endothelial cells. A limited number of patterns of lysis of one, or more than one, of the four different target cells was observed. The nature of the possible target determinants recognized by these CTL-clones is discussed.
关于同种异体移植排斥反应中所识别抗原的知识仍不完整。利用克隆的细胞毒性T淋巴细胞来研究犬体内靶抗原决定簇的分布。通过有限稀释技术从混合淋巴细胞培养产生的效应细胞中获得细胞毒性T淋巴细胞克隆。已对这些克隆针对PHA刺激的淋巴母细胞、单核细胞以及动脉和静脉内皮细胞的细胞介导细胞毒性进行了检测。观察到对四种不同靶细胞中的一种或多种细胞的有限裂解模式。讨论了这些细胞毒性T淋巴细胞克隆所识别的可能靶抗原决定簇的性质。
