Groenewegen G, Buurman W A, van der Linden C J, Jeunhomme G M, Kootstra G
Tissue Antigens. 1983 Feb;21(2):114-28.
A method is described which permits culture of both arterial and venous canine endothelial cells. Cell-mediated cytotoxicity against cultured endothelial cells has been studied. A 51Cr-release assay was used to detect CTL generated in MLC. Both arterial and venous endothelial cells are lysed by CTL specifically. Cold target inhibition experiments have been performed to analyse the CTL-recognized antigens on arterial and venous endothelial cells. Different antigens are recognized by CTL on venous endothelial cells and PHA-blasts; it is possible that CTL recognize venous endothelial cells through class II antigens or E-M antigens. Arterial endothelial cells and PHA-blasts share CTL-recognized antigens.
本文描述了一种可用于培养犬动脉和静脉内皮细胞的方法。已对针对培养内皮细胞的细胞介导细胞毒性进行了研究。采用51Cr释放试验检测混合淋巴细胞培养中产生的细胞毒性T淋巴细胞（CTL）。动脉和静脉内皮细胞均可被CTL特异性裂解。已进行冷靶抑制实验以分析动脉和静脉内皮细胞上CTL识别的抗原。CTL在静脉内皮细胞和PHA刺激的淋巴细胞上识别不同的抗原；CTL可能通过II类抗原或E-M抗原识别静脉内皮细胞。动脉内皮细胞和PHA刺激的淋巴细胞共享CTL识别的抗原。
