Devan Shobana Mukunda, Pailoor Jayalakshmi, Sthaneshwar Pavai, Narayanan Vallikkanu
Pathology Department, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia E-mail :
Asian Pac J Cancer Prev. 2013;14(8):4545-8. doi: 10.7314/apjcp.2013.14.8.4545.
The objective of this study is to assess tissue expression of CA-125 and HE4 protein in primary benign and malignant epithelial tumours of the ovary and correlate with serum CA-125 levels. A total of 100 formalin-fixed, paraffin embedded sections of ovarian tumours which included serous adenoma (11), mucinous adenoma (42), serous carcinoma (20), mucinous carcinoma (12) and endometrioid carcinoma (15), histologically diagnosed between 1st January 2004 to 31st December 2012 at the University Malaya Medical Centre, were stained for HE4 (rabbit polyclonal antibody, Abcam, UK) and CA-125 (mouse monoclonal antibody clone: OC125, Cell Marque Corporation, Rocklin, California, USA). Pre-operative serum CA-125 levels were obtained from the laboratory information system. Immunoscore (I score) for HE4 and CA-125 was given based on the intensity of staining and percentage of positive tumour cells and considered significant when it was >50 (intensity of staining multiplied by percentage of positive tumour cells). Serum CA-125 levels were compared with the I score of HE4 and CA-125 in tissues. We noted that the CA-125 levels in serum and tissues were significantly raised in malignant compared to benign ovarian tumours (p value<0.05). Tissue expression of HE4 protein was also significantly raised in malignant tumours compared to benign tumours (p value<0.05). We conclude that HE4 can be a useful tissue immunomarker in addition to CA-125.
本研究的目的是评估CA - 125和HE4蛋白在原发性卵巢良性和恶性上皮性肿瘤中的组织表达，并与血清CA - 125水平进行相关性分析。2004年1月1日至2012年12月31日期间在马来亚大学医学中心经组织学诊断的100例卵巢肿瘤的福尔马林固定石蜡包埋切片，包括浆液性腺瘤（11例）、黏液性腺瘤（42例）、浆液性癌（20例）、黏液性癌（12例）和子宫内膜样癌（15例），用HE4（兔多克隆抗体，英国Abcam公司）和CA - 125（小鼠单克隆抗体克隆号：OC125，美国加利福尼亚州罗克林市Cell Marque公司）进行染色。术前血清CA - 125水平从实验室信息系统获取。根据染色强度和阳性肿瘤细胞百分比给予HE4和CA - 125免疫评分（I评分），当I评分>50（染色强度乘以阳性肿瘤细胞百分比）时认为具有显著性。将血清CA - 125水平与组织中HE4和CA - 125的I评分进行比较。我们注意到，与良性卵巢肿瘤相比，恶性肿瘤血清和组织中的CA - 125水平显著升高（p值<0.05）。与良性肿瘤相比，恶性肿瘤中HE4蛋白的组织表达也显著升高（p值<0.05）。我们得出结论：除CA - 125外，HE4也可作为一种有用的组织免疫标志物。
