CA-125 靶向纳米泡对比剂在卵巢癌的超声分子成像。
Ultrasound molecular imaging of ovarian cancer with CA-125 targeted nanobubble contrast agents.
机构信息
Department of Ultrasound, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; Department of Radiology, Case Center for Imaging Research, Case Western Reserve University, Cleveland, OH, USA.
Department of Radiology, Case Center for Imaging Research, Case Western Reserve University, Cleveland, OH, USA.
出版信息
Nanomedicine. 2017 Oct;13(7):2159-2168. doi: 10.1016/j.nano.2017.06.001. Epub 2017 Jun 9.
Ultrasound is frequently utilized in diagnosis of gynecologic malignancies such as ovarian cancer. Because epithelial ovarian cancer (EOC) is often characterized by overexpression of cancer antigen 125 (CA-125), ultrasound contrast agents able to target this molecular signature could be a promising complementary strategy. In this work, we demonstrate application of CA-125-targeted echogenic lipid and surfactant-stabilized nanobubbles imaged with standard clinical contrast harmonic ultrasound for imaging of CA-125 positive OVCAR-3 tumors in mice. Surface functionalization of the nanobubbles with a CA-125 antibody achieved rapid significantly (P < 0.05) enhanced tumor accumulation, higher peak ultrasound signal intensity and slower wash out rates in OVCAR-3 tumors compared to CA-125 negative SKOV-3 tumors. Targeted nanobubbles also exhibited increased tumor retention and prolonged echogenicity compared to untargeted nanobubbles. Data suggest that ultrasound molecular imaging using CA-125 antibody-conjugated nanobubbles may contribute to improved diagnosis of EOC.
超声在妇科恶性肿瘤（如卵巢癌）的诊断中经常被使用。由于上皮性卵巢癌（EOC）通常表现为癌抗原 125（CA-125）的过度表达，因此能够针对这种分子特征的超声造影剂可能是一种很有前途的补充策略。在这项工作中，我们展示了用标准临床对比谐波超声成像的 CA-125 靶向声振脂质和表面活性剂稳定纳米气泡在 CA-125 阳性 OVCAR-3 荷瘤小鼠中的应用。纳米气泡表面用 CA-125 抗体进行功能化，与 CA-125 阴性 SKOV-3 肿瘤相比，在 OVCAR-3 肿瘤中实现了快速显著（P < 0.05）增强的肿瘤积累、更高的峰值超声信号强度和更慢的洗脱率。与未靶向的纳米气泡相比，靶向纳米气泡还表现出增加的肿瘤保留和延长的超声增强时间。数据表明，使用 CA-125 抗体偶联纳米气泡的超声分子成像可能有助于改善 EOC 的诊断。
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