Zhirnov O P, Ovcharenko A V, Bukrinskaia A G
Vopr Virusol. 1985 Mar-Apr;30(2):207-14.
The mandatory step in reproduction of myxoviruses (influenza viruses and paramyxoviruses) is proteolytic shearing of viral glycoproteins activating the infectivity of virions. Such activation of myxoviruses is realized by trypsin-like proteases of the host. This study demonstrated that proteolytic activation of virions could be inhibited by a physiological inhibitor of proteases, aprotinine. A single injection of aprotinine (preparations Gordox or Contrycal) into chick embryos infected with various influenza viruses (WSN/34, Udorn/72) and paramyxoviruses (Sendai/960, NDV/La Sota, NDV/Queensland) blocked shearing of viral glycoproteins, HA, FO, HNO as a result of which noninfectious virions with unsheared glycoproteins were predominantly synthesized. In aprotinine-treated embryos, multicycle virus infection was markedly decreased which led to the 10(4)-fold or greater reduction of the virus yield. The antiviral effect of protease inhibitors and possibilities of their practical use in viral diseases are discussed.
黏液病毒（流感病毒和副黏病毒）复制过程中的一个必要步骤是病毒糖蛋白的蛋白水解切割，从而激活病毒粒子的感染性。黏液病毒的这种激活是由宿主的类胰蛋白酶实现的。本研究表明，蛋白酶的生理抑制剂抑肽酶可以抑制病毒粒子的蛋白水解激活。向感染了各种流感病毒（WSN/34、乌东/72）和副黏病毒（仙台/960、新城疫病毒/La Sota、新城疫病毒/昆士兰）的鸡胚中单次注射抑肽酶（商品名Gordox或Contrycal），可阻断病毒糖蛋白HA、FO、HNO的切割，结果主要合成了糖蛋白未被切割的无感染性病毒粒子。在经抑肽酶处理的鸡胚中，多轮病毒感染显著减少，这导致病毒产量降低了10⁴倍或更多。文中讨论了蛋白酶抑制剂的抗病毒作用及其在病毒性疾病中的实际应用可能性。
