Pediatric Endocrinology Department, Shiraz University of Medical Sciences, Shiraz, Iran.
J Endocrinol Invest. 2013 Dec;36(11):1090-3. doi: 10.3275/9098. Epub 2013 Sep 27.
Failure in self-tolerance towards β-cells in diabetes mellitus (DM) pathogenesis involves a series of complex events that are governed by environmental and genetic factors. Considering the importance of osteopontin (OPN) in T-helper-1 (Th1) cells development, the aim of this study was to evaluate the serum level and gene polymorphism of OPN in Iranian Type 1 diabetic (T1DM) children.
In this case-control study, 87 T1DM children and 86 healthy ones were enrolled. Blood samples of both groups were checked for OPN level. The single nucleotide polymorphisms (SNP) were genotyped by RFLP analysis for OPN rs1126772, its receptor integrin α4 (ITGA4) rs 1449263, and CD44 rs8193.
Serum levels of OPN in diabetic children were significantly higher in cases compared to the control group (p=0.023), but there was no significant relationship between OPN rs1126772 (p=0.79), its receptor integrin α4 (p=0.31), and CD44 rs8193 (p=0.45), and T1DM.
Higher amounts of OPN were seen in T1DM children. It is assumed that OPN might have inducing effects on T1DM development, particularly when genetically susceptible individuals are predisposed by an environmental insult. However, the 3 SNPs of OPN and its receptors did not show noticeable association with T1DM. The power of our study (~19%) was insufficient to observe any significant statistical difference between the groups; moreover, this study does not exclude the possibility of association of other SNPs of OPN and its receptors with this disease, and more studies are needed to clarify the issue.
糖尿病（DM）发病机制中对β细胞的自身耐受失败涉及一系列复杂事件，这些事件受环境和遗传因素的控制。考虑到骨桥蛋白（OPN）在辅助性 T 细胞 1（Th1）细胞发育中的重要性，本研究旨在评估伊朗 1 型糖尿病（T1DM）儿童的血清 OPN 水平和基因多态性。
在这项病例对照研究中，纳入了 87 名 T1DM 儿童和 86 名健康对照者。检测两组的血液样本以检查 OPN 水平。采用 RFLP 分析检测 OPN rs1126772、其受体整合素 α4（ITGA4）rs1449263 和 CD44 rs8193 的单核苷酸多态性（SNP）。
与对照组相比，糖尿病患儿的血清 OPN 水平显著升高（p=0.023），但 OPN rs1126772（p=0.79）、其受体整合素 α4（p=0.31）和 CD44 rs8193（p=0.45）与 T1DM 之间无显著相关性。
T1DM 儿童中 OPN 含量较高。可以假设 OPN 可能对 T1DM 的发展具有诱导作用，特别是在易感个体受到环境损害时。然而，OPN 及其受体的 3 个 SNP 与 T1DM 无明显相关性。我们研究的效力（~19%）不足以观察到两组之间的任何显著统计学差异；此外，本研究不能排除 OPN 及其受体的其他 SNPs 与该疾病相关的可能性，需要更多的研究来阐明这个问题。
