Lavi Hadas, Assayag Miri, Schwartz Assaf, Arish Nissim, Fridlender Zvi G, Berkman Neville
Institute of Pulmonary Medicine, Hebrew University Hadassah Medical Center, Jerusalem, Israel.
PLoS One. 2017 Mar 2;12(3):e0171945. doi: 10.1371/journal.pone.0171945. eCollection 2017.
Sarcoidosis is a systemic inflammatory disease of unknown etiology. Osteopontin (SPP1, OPN) is an extra cellular matrix glycoprotein and cytokine with a known role in granuloma formation and in autoimmune and inflammatory diseases.
To determine whether plasma OPN levels are elevated in patients with sarcoidosis and compare the frequency of four single nucleotide polymorphism (SNPs) variants in the OPN gene in sarcoidosis patients compared to healthy controls.
Demographic and clinical information, radiological studies and pulmonary function tests were evaluated in 113 patients with sarcoidosis and in 79 healthy controls. Blood samples were analyzed for SNPs of the OPN gene and for plasma OPN and CRP levels. Association between clinical features of disease and OPN levels as well as SNP frequencies was determined.
Plasma OPN levels were higher in sarcoidosis patients than in healthy subjects, (median: 217 vs 122ng/ml, p<0.001). Area under the curve for receiver operator curves (ROC) was 0.798 (0.686-0.909 95% CI.) No differences were observed between sarcoidosis patients and controls in the frequency of any of the SNPs evaluated. Presence of lung parenchymal involvement was associated with SNP distribution at rs1126772 (p = 0.02). We found no correlation between SNPs distribution and plasma OPN levels.
Osteopontin protein levels are elevated in sarcoidosis. We found no evidence for an association between SNPs on the osteopontin gene and plasma OPN levels or the presence of sarcoidosis, however, an association between genotype and several phenotypic clinical parameters of disease was observed.
结节病是一种病因不明的全身性炎症性疾病。骨桥蛋白（SPP1，OPN）是一种细胞外基质糖蛋白和细胞因子，在肉芽肿形成以及自身免疫和炎症性疾病中具有已知作用。
确定结节病患者血浆OPN水平是否升高，并比较结节病患者与健康对照者OPN基因中四种单核苷酸多态性（SNP）变体的频率。
对113例结节病患者和79例健康对照者的人口统计学和临床信息、放射学研究及肺功能测试进行评估。分析血样中的OPN基因SNP以及血浆OPN和CRP水平。确定疾病临床特征与OPN水平以及SNP频率之间的关联。
结节病患者的血浆OPN水平高于健康受试者（中位数：217对122ng/ml，p<0.001）。受试者操作曲线（ROC）的曲线下面积为0.798（0.686 - 0.909，95%CI）。在评估的任何SNP频率方面，结节病患者与对照者之间未观察到差异。肺实质受累与rs1126772处的SNP分布相关（p = 0.02）。我们发现SNP分布与血浆OPN水平之间无相关性。
结节病中骨桥蛋白水平升高。我们未发现骨桥蛋白基因SNP与血浆OPN水平或结节病存在之间存在关联的证据，然而，观察到基因型与疾病的几个表型临床参数之间存在关联。
