Department of Biological Engineering, Utah State University, UT 84322, United States.
Bioresour Technol. 2013 Dec;149:65-70. doi: 10.1016/j.biortech.2013.09.028. Epub 2013 Sep 17.
The potential of microalgae as a source of sustainable energy, nutritional supplements and specialized chemicals necessitates a thorough evaluation of the methods of harvesting microalgae with regards to the bioproduct(s) desired. This research assessed the effect of coagulation, flocculation, and centrifugation on the wet lipid extraction procedure, which fractionated microalgae into hydrolyzed biomass for fermentation into acetone, butanol, and ethanol, an aqueous phase as growth media for genetically engineered Escherichia coli, and a lipid fraction for the production of biodiesel. Biomass harvested by cationic starches, alum, and centrifugation produced 30, 19, and 22.5mg/g of dry wt. algae of total combined acetone, butanol, and ethanol, respectively. Higher biodiesel production was also observed for the cationic starches (9.6 mg/g of dry wt. algae) than alum (0.6 mg/g of dry wt. algae) harvested biomass. The results suggested significant effect of the harvesting methods on the yields of bioproducts.
微藻作为可持续能源、营养补充剂和特种化学品的潜在来源，需要根据所需的生物制品，对微藻的收获方法进行全面评估。本研究评估了凝聚、絮凝和离心对湿脂质提取程序的影响，该程序将微藻分离成水解生物质，用于发酵丙酮、丁醇和乙醇，将水相作为遗传工程大肠杆菌的生长培养基，以及用于生产生物柴油的脂质部分。用阳离子淀粉、明矾和离心法收获的生物质分别产生 30、19 和 22.5mg/g 的干重藻类，分别为丙酮、丁醇和乙醇的总结合物。与明矾（干重藻类 0.6mg/g）收获的生物质相比，阳离子淀粉（干重藻类 9.6mg/g）的生物柴油产量也更高。结果表明，收获方法对生物制品的产量有显著影响。
