Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, Qingdao, China.
Bioresour Technol. 2013 Dec;149:77-83. doi: 10.1016/j.biortech.2013.09.027. Epub 2013 Sep 18.
The Antarctic ice microalgae Chlamydomonas sp. ICE-L which is highly resistant to salt stress holds promise in providing an alternative species for the production of microalgal oil. We studied the effects of the alga in confrontation with NaCl stress on the growth, oil yield and expression of fatty acid desaturase genes. The growth rate of Chlamydomonas sp. ICE-L decreased with the gradual increase in NaCl concentration. Interestingly, we found that the highest lipid content was achieved at 16‰ NaCl, reaching 23% (w/w). Meanwhile, the expression of Δ9ACPCiFAD increased rapidly while Δ12CiFAD, ω3CiFAD2 and Δ6CiFAD showed a delayed elevation in response to altered salt stress. C18:3 was the dominant PUFA, which account for about 75% TFA in Chlamydomonas sp. ICE-L. Under 96‰ and 128‰ NaCl stress, the content of C20:5 almost approached that of C18:3. In contrast, low salinity enhanced the dominance of C18:3 at the expense of C20:3 and C20:5.
耐盐性极强的南极冰藻 Chlamydomonas sp. ICE-L 有望成为生产微藻油的替代物种。我们研究了该藻类在应对 NaCl 胁迫时对生长、产油量和脂肪酸去饱和酶基因表达的影响。Chlamydomonas sp. ICE-L 的生长速率随着 NaCl 浓度的逐渐增加而降低。有趣的是，我们发现，在 16‰ NaCl 时达到最高的脂质含量，达到 23%（w/w）。同时，Δ9ACPCiFAD 的表达迅速增加，而 Δ12CiFAD、ω3CiFAD2 和 Δ6CiFAD 则表现出对盐胁迫改变的延迟升高。C18:3 是主要的多不饱和脂肪酸（PUFA），占 Chlamydomonas sp. ICE-L 总脂肪酸（TFA）的约 75%。在 96‰和 128‰ NaCl 胁迫下，C20:5 的含量几乎接近 C18:3。相比之下，低盐度增强了 C18:3 的优势，而牺牲了 C20:3 和 C20:5。
