Hounslow Emily, Kapoore Rahul Vijay, Vaidyanathan Seetharaman, Gilmour D James, Wright Phillip C
ChELSI Institute, Department of Chemical and Biological Engineering, Faculty of Engineering, The University of Sheffield, Sheffield, UK.
Department of Molecular Biology and Biotechnology, Faculty of Science, The University of Sheffield, Sheffield, UK.
Curr Biotechnol. 2016 Nov;5(4):305-313. doi: 10.2174/2211550105666160322234434.
Algal cells produce neutral lipid when stressed and this can be used to generate biodiesel.
Salt stressed cells of the model microalgal species Chlamydomonas reinhardtii were tested for their suitability to produce lipid for biodiesel.
The starchless mutant of C. reinhardtii (CC-4325) was subjected to salt stress (0.1, 0.2 and 0.3 M NaCl) and transesterification and GC analysis were used to determine fatty acid methyl ester (FAME) content and profile.
Fatty acid profile was found to vary under salt stress conditions, with a clear distinction between 0.1 M NaCl, which the algae could tolerate, and the higher levels of NaCl (0.2 and 0.3 M), which caused cell death. Lipid content was increased under salt conditions, either through long-term exposure to 0.1 M NaCl, or short-term exposure to 0.2 and 0.3 M NaCl. Palmitic acid (C16:0) and linolenic acid (C18:3n3) were found to increase significantly at the higher salinities.
Salt increase can act as a lipid trigger for C. reinhardtii.
藻类细胞在受到胁迫时会产生中性脂质，可用于生产生物柴油。
测试模式微藻莱茵衣藻经盐胁迫处理后的细胞用于生产生物柴油脂质的适用性。
对莱茵衣藻的无淀粉突变体（CC-4325）施加盐胁迫（0.1、0.2和0.3M氯化钠），并采用酯交换反应和气相色谱分析来测定脂肪酸甲酯（FAME）的含量和分布。
发现盐胁迫条件下脂肪酸分布存在差异，藻类能够耐受的0.1M氯化钠与导致细胞死亡的较高浓度氯化钠（0.2和0.3M）之间有明显区别。在盐胁迫条件下，通过长期暴露于0.1M氯化钠或短期暴露于0.2和0.3M氯化钠，脂质含量均有所增加。在较高盐度下，棕榈酸（C16:0）和亚麻酸（C18:3n3）显著增加。
盐浓度增加可作为莱茵衣藻脂质合成的触发因素。
