Huber Carola, Anand A Alwin Prem, Mauz Manfred, Künstle Peter, Hupp Wolfgang, Hirt Bernhard, Wizenmann Andrea
Department of Experimental Embryology, Institute of Anatomy, University of Tübingen.
J Vis Exp. 2013 Sep 16(79):e50024. doi: 10.3791/50024.
Non-coding RNAs are additional players in regulating gene expression. Targeted in ovo electroporation of specific areas provides a unique tool for spatial and temporal control of ectopic microRNA expression. However, ventral brain structures like ventral midbrain are rather difficult to reach for any manipulations. Here, we demonstrate an efficient way to electroporate miRNA into ventral midbrain using thin platinum electrodes. This method offers a reliable way to transfect specific areas of the midbrain and a useful tool for in vivo studies.
非编码RNA是基因表达调控中的其他参与者。在特定区域进行卵内靶向电穿孔为异位微小RNA表达的时空控制提供了一种独特的工具。然而，像腹侧中脑这样的脑腹侧结构很难进行任何操作。在这里，我们展示了一种使用细铂电极将微小RNA电穿孔到腹侧中脑的有效方法。该方法为转染中脑的特定区域提供了一种可靠的方式，也是体内研究的一种有用工具。
