Calvert R, Schneeman B O, Satchithanandam S, Cassidy M M, Vahouny G V
Am J Clin Nutr. 1985 Jun;41(6):1249-56. doi: 10.1093/ajcn/41.6.1249.
Male Wistar rats were fed for four weeks on defined diets containing no fiber additions, 10% levels of insoluble fiber derivatives (cellulose or alfalfa), or 5% levels of viscous fiber derivatives (pectin, guar gum, or metamucil). After an overnight fast, the pancreas was assayed for protein, amylase, lipase, trypsin, and chymotrypsin. Homogenates of small intestinal mucosa were analyzed for protein, alkaline phosphatase, invertase and thymidine kinase. There were, with few exceptions, no dietary effects on the exocrine pancreatic enzymes. The specific activities of the villus marker enzymes (invertase and alkaline phosphatase) tended to be higher in the proximal (but not middle or distal) intestines of the fiber-fed groups, while total activities were the same in all groups. In contrast, the activity of the crypt marker, thymidine kinase, was highest in the distal intestinal segments, and even higher in animals given the alfalfa, guar gum or metamucil-supplemented diets.
将雄性Wistar大鼠分别饲喂含以下成分的特定日粮四周：不添加纤维、10%水平的不溶性纤维衍生物（纤维素或苜蓿）或5%水平的粘性纤维衍生物（果胶、瓜尔豆胶或甲基纤维素）。禁食过夜后，检测胰腺中的蛋白质、淀粉酶、脂肪酶、胰蛋白酶和糜蛋白酶。分析小肠黏膜匀浆中的蛋白质、碱性磷酸酶、转化酶和胸苷激酶。除少数例外情况外，日粮对胰腺外分泌酶没有影响。纤维喂养组近端（而非中段或远端）小肠中绒毛标记酶（转化酶和碱性磷酸酶）的比活性往往较高，而所有组的总活性相同。相比之下，隐窝标记物胸苷激酶的活性在远端肠段最高，在给予苜蓿、瓜尔豆胶或甲基纤维素补充日粮的动物中甚至更高。
