Jensen M S, Jensen S K, Jakobsen K
Danish Institute of Animal Science, Department of Nutrition, Research Centre Foulum, Tjele, Denmark.
J Anim Sci. 1997 Feb;75(2):437-45. doi: 10.2527/1997.752437x.
The effect of age and weaning on the activities of digestive enzymes with emphasis on the lipolytic enzymes before and after weaning was investigated. The activities of amylase, chymotrypsin, trypsin, carboxyl ester hydrolase, pancreatic lipase, and colipase in pancreatic tissue and the activity of gastric lipase in the cardiac mucosa of the stomach in 45 pigs were response variables. The activity of trypsin was not affected by weaning and the rate of increase was similar during the whole experiment. The activities of chymotrypsin and amylase decreased at weaning (P < .05). After weaning the activity of chymotrypsin increased more slowly than before weaning (P < .001), whereas the rate of increase of amylase activity remained unchanged. Lipase, colipase, and carboxyl ester hydrolase activities decreased at weaning (P < .001), whereas gastric lipase activity increased at weaning (P < .01). The development of lipase, colipase, and carboxyl ester hydrolase activity decreased postweaning (P < .01), whereas gastric lipase activity increased before weaning and remained constant after weaning. Pancreatic lipase had a considerably higher capacity for hydrolyzing tributyrin, and the total activity of pancreatic lipase was up to 600 times higher than that of gastric lipase. The lipolytic enzymes displayed a non-parallel pattern of development, and we suggest that this reflects the importance of these enzymes during the suckling and postweaning phases, respectively. However, the significance of gastric lipase for the digestion of fat in pigs remains to be elucidated.
研究了年龄和断奶对消化酶活性的影响，重点关注断奶前后的脂解酶。45头猪胰腺组织中淀粉酶、胰凝乳蛋白酶、胰蛋白酶、羧基酯水解酶、胰腺脂肪酶和辅脂肪酶的活性以及胃贲门黏膜中胃脂肪酶的活性为反应变量。胰蛋白酶的活性不受断奶影响，在整个实验过程中增加速率相似。胰凝乳蛋白酶和淀粉酶的活性在断奶时降低（P <.05）。断奶后，胰凝乳蛋白酶的活性增加比断奶前更慢（P <.001），而淀粉酶活性的增加速率保持不变。脂肪酶、辅脂肪酶和羧基酯水解酶的活性在断奶时降低（P <.001），而胃脂肪酶的活性在断奶时增加（P <.01）。断奶后，脂肪酶、辅脂肪酶和羧基酯水解酶活性的发育降低（P <.01），而胃脂肪酶的活性在断奶前增加，断奶后保持恒定。胰腺脂肪酶水解三丁酸甘油酯的能力相当高，胰腺脂肪酶的总活性比胃脂肪酶高600倍。脂解酶呈现出非平行的发育模式，我们认为这分别反映了这些酶在哺乳和断奶后阶段的重要性。然而，胃脂肪酶对猪脂肪消化的重要性仍有待阐明。
