National Research Institute for Family Planning.
Eur J Histochem. 2013 Sep 27;57(3):e25. doi: 10.4081/ejh.2013.e25.
Cyclic shedding of the endometrium is unique to menstruating species. The status of the decidua in mouse menstrual-like models seems to differ from that of the predecidua in humans before endometrial breakdown. The aim of this study was to determine how this difference in decidual status is related to endometrial breakdown. A mouse menstrual-like model was generated by pharmacological progesterone withdrawal. Histomorphological analysis and reticular fiber staining were used to evaluate endometrial status. In situ zymography was used to determine the localization of active collagenase and gelatinase. The functional endometrial layer containing the mature decidual-like zone (MDZ) and predecidual-like zone (PZ) underwent breakdown. The reticular fibers underwent disruption and fragmentation and became loose or disappeared at 12 h in the PZ, where active collagenase and gelatinase were limited. The reticular fibers were visibly reduced at 24 h in the MDZ, where active collagenase was detected. A few reticular fibers remained; however, the functional layer had sloughed into the lumen of the uterus. The results showed that reticular fibers of the PZ are actively degraded during endometrial shedding.
周期性的子宫内膜脱落是月经物种所特有的。在类似于月经的小鼠模型中，蜕膜的状态似乎与人类子宫内膜破裂前的前置蜕膜不同。本研究旨在确定这种蜕膜状态的差异与子宫内膜破裂的关系。通过药理学孕酮撤退来建立类似于月经的小鼠模型。组织形态学分析和网状纤维染色用于评估子宫内膜状态。原位酶谱法用于确定活性胶原酶和明胶酶的定位。含有成熟蜕膜样区（MDZ）和前置蜕膜样区（PZ）的功能性子宫内膜层发生破裂。在 PZ 中，网状纤维在 12 小时时发生断裂和碎片化，变得松散或消失，活性胶原酶和明胶酶受到限制。在 MDZ 中，在检测到活性胶原酶的 24 小时时，网状纤维明显减少。尽管仍有少量网状纤维存在，但功能层已脱落到子宫腔中。结果表明，在子宫内膜脱落过程中，PZ 的网状纤维被积极降解。
