Roy A K, Sarkar J, Bhadra R, Datta A G
Arch Biochem Biophys. 1985 Jun;239(2):364-7. doi: 10.1016/0003-9861(85)90700-3.
L-Epinephrine, serotonin, and isoproterenol stimulate the incorporation of [14C]leucine into thrombin-induced clottable protein; this stimulation was abolished by actinomycin D. The incorporation of 32P into total RNA of rat liver, the site of fibrinogen synthesis, was stimulated by epinephrine and was highest at 2 h after 32P administration. [14C]Orotic acid incorporation into polysomal RNA of liver was also increased significantly by epinephrine and serotonin. The immunoprecipitation of newly synthesized protein by monospecific antibody raised against pure rat fibrinogen clearly demonstrates that L-epinephrine increased fibrinogen formation in vivo under the experimental condition. Translation of poly (A)-containing RNA from total polysomal RNA clearly indicates that L-epinephrine increased mRNA specific for fibrinogen.
L-肾上腺素、血清素和异丙肾上腺素可刺激[14C]亮氨酸掺入凝血酶诱导的可凝蛋白中；这种刺激作用被放线菌素D消除。肾上腺素可刺激32P掺入大鼠肝脏（纤维蛋白原合成部位）的总RNA中，且在给予32P后2小时达到最高值。肾上腺素和血清素也显著增加了[14C]乳清酸掺入肝脏多聚核糖体RNA中的量。用针对纯大鼠纤维蛋白原产生的单特异性抗体对新合成的蛋白质进行免疫沉淀，清楚地表明在实验条件下，L-肾上腺素可在体内增加纤维蛋白原的形成。从总多聚核糖体RNA中翻译含多聚腺苷酸（poly(A)）的RNA清楚地表明，L-肾上腺素增加了纤维蛋白原特异性的信使核糖核酸（mRNA）。
