Murayama M, Hikino H
J Ethnopharmacol. 1984 Oct;12(1):25-33. doi: 10.1016/0378-8741(84)90085-0.
Mesaconitine (MA) significantly stimulated the incorporation of 5-[3H]-orotic acid into liver nuclear RNA 16 h after its administration. MA exhibited the strongest activity among the aconitine alkaloids. This stimulatory effect of MA was inhibited by actinomycin D, but the incorporation ratio of 5-[3H]orotic acid in the combined treatment group with MA and actinomycin D was significantly higher than that in the single treatment group with actinomycin D. MA also increased the incorporation of 5-[3H]orotic acid into polysomal RNA in mouse liver. When MA was added to the RNA polymerase (EC 2.7.7.6) preparation obtained from rat liver, the increase of the enzyme activity was weak. While RNA polymerase preparation from the liver of rats, which were previously treated with MA, elicited increased incorporation of [3H]cytidine monophosphate into RNA as compared with that from the livers of normal rats. Accumulated data indicate that aconitine alkaloids, in particular MA, accelerate liver RNA synthesis mainly by the increase of RNA polymerase.
中乌头碱（MA）给药16小时后，能显著刺激5-[³H]-乳清酸掺入肝细胞核RNA。在乌头碱类生物碱中，MA表现出最强的活性。MA的这种刺激作用可被放线菌素D抑制，但MA与放线菌素D联合治疗组中5-[³H]乳清酸的掺入率显著高于放线菌素D单药治疗组。MA还增加了5-[³H]乳清酸掺入小鼠肝脏多聚核糖体RNA。当将MA添加到从大鼠肝脏获得的RNA聚合酶（EC 2.7.7.6）制剂中时，酶活性的增加较弱。而与正常大鼠肝脏相比，先前用MA处理过的大鼠肝脏的RNA聚合酶制剂使[³H]胞苷单磷酸掺入RNA的量增加。积累的数据表明，乌头碱类生物碱，特别是MA，主要通过增加RNA聚合酶来加速肝脏RNA合成。
