Sekiya S, Mogi M, Tanigawa S, Hayashi H, Uchiyama T, Okayasu M
Gan To Kagaku Ryoho. 1985 Mar;12(3 Pt 1):524-9.
We tried an in vitro sensitivity test for the purpose of examining the effect of anti-neoplastic agents and their enhancement by Biscolaurine Alkaloid (Cepharanthin). Methods, HeLa cells were synchronized by excess of thymidine and hydroxyurea, and resuspended in Eagle's MEM with 10% FCS. The cells were mixed with Mitomycin C or Bleomycin and added to the wells of a microtiter plate. When required, Cepharanthin (the effect of this drug is considered to change the plasma membrane liquidity) was added to the medium, and then cultured for 24 hours in a CO2 incubator. After culture, each well received 3H-thymidine, and was incubated for 8 hours. The cells were harvested for the assay of 3H-thymidine.
MMC or BLM suppressed the uptake of 3H-thymidine in relation to its concentration. MMC or BLM with various concentrations of Cepharanthin showed strong suppression compared with the same concentrations of MMC or BLM alone. From the above results, this experimental method is regarded as an useful model for selecting sensitive anti-neoplastic agents. In addition, Cepharanthin showed enhancing effects on anti-neoplastic agents.
我们进行了一项体外敏感性试验，以研究抗肿瘤药物的效果以及双氢小檗碱生物碱（千金藤素）对其的增强作用。方法，用过量的胸苷和羟基脲使HeLa细胞同步化，然后重悬于含10%胎牛血清的伊格尔氏MEM培养基中。将细胞与丝裂霉素C或博来霉素混合，并加入微量滴定板的孔中。如有需要，将千金藤素（认为该药物的作用是改变质膜流动性）加入培养基中，然后在二氧化碳培养箱中培养24小时。培养后，每个孔加入3H-胸苷，并孵育8小时。收获细胞用于检测3H-胸苷。
丝裂霉素C或博来霉素对3H-胸苷摄取的抑制作用与其浓度相关。与单独使用相同浓度的丝裂霉素C或博来霉素相比，添加不同浓度千金藤素的丝裂霉素C或博来霉素显示出更强的抑制作用。根据上述结果，该实验方法被认为是筛选敏感抗肿瘤药物的有用模型。此外，千金藤素对抗肿瘤药物有增强作用。
