Krivonosov S K, Zorin N A, Ionova N K, Terent'ev A A, Tatarinov Iu S
Biull Eksp Biol Med. 1985 May;99(5):568-71.
It has been established that trophoblast-specific beta-glycoprotein and pregnancy-associated alpha 2-glycoprotein specifically react with non-fixed PHA and Con A. Affinity to the former protein is significantly higher than to the latter one. alpha-Fetoprotein has a low affinity to Con A alone. Affinity to this lectin is in an agreement with the content of carbohydrates contained by pregnancy proteins. A considerable part of human serum proteins bind with Con A; receptors for PHA possess only some serum proteins. As the above-mentioned lectins are often used for stimulation of lymphocyte blast transformation, in is recommended that the constituent parts of the culture medium should be preliminarily tested to specify more accurately their affinity to PHA and Con A.
现已确定，滋养层特异性β-糖蛋白和妊娠相关α2-糖蛋白能与非固定化的PHA和Con A发生特异性反应。与前一种蛋白的亲和力明显高于后一种。甲胎蛋白单独与Con A的亲和力较低。与这种凝集素的亲和力与妊娠蛋白所含碳水化合物的含量一致。相当一部分人血清蛋白能与Con A结合；PHA的受体仅存在于某些血清蛋白中。由于上述凝集素常被用于刺激淋巴细胞的胚细胞转化，建议对培养基的组成成分进行预先检测，以更准确地确定它们与PHA和Con A的亲和力。
