人迁移抑制因子的抗原依赖性异质性。

Antigen-dependent heterogeneity of human migration inhibitory factor.

作者信息

Weiser W Y, David J R, Remold H G

出版信息

Cell Immunol. 1985 Jul;93(2):532-40. doi: 10.1016/0008-8749(85)90157-1.

Abstract

Human migration inhibitory factor (MIF) produced by peripheral blood mononuclear cells stimulated with purified protein derivative, tetanus toxoid, streptokinase-streptodornase, or Candida albicans antigen was analyzed by gel filtration and isoelectrofocusing. In all cases, supernatants harvested after a 24-hr exposure of the mononuclear cells to the antigen yielded only one MIF species with an isoelectric point of 5. In contrast, isoelectrofocusing of supernatants obtained from cells exposed to the antigen for an additional 24 hr demonstrated that different antigens induce the elaboration of different MIF species. Streptokinase-streptodornase and tetanus toxoid induced the production of one MIF species with an isoelectric point of 5 (pH 5-MIF). Stimulation of cells with Candida antigen elaborated a MIF species with an isoelectric point of 3 (pH 3-MIF). In contrast, stimulation of cells with purified protein derivative induced the production of both pH 3-MIF and pH 5-MIF.

摘要

采用凝胶过滤和等电聚焦法分析了经纯化蛋白衍生物、破伤风类毒素、链激酶-链道酶或白色念珠菌抗原刺激的外周血单核细胞产生的人迁移抑制因子（MIF）。在所有情况下，单核细胞与抗原接触24小时后收获的上清液仅产生一种等电点为5的MIF。相比之下，对与抗原再接触24小时的细胞获得的上清液进行等电聚焦分析表明，不同抗原诱导产生不同的MIF。链激酶-链道酶和破伤风类毒素诱导产生一种等电点为5的MIF（pH 5-MIF）。用念珠菌抗原刺激细胞产生一种等电点为3的MIF（pH 3-MIF）。相反，用纯化蛋白衍生物刺激细胞会产生pH 3-MIF和pH 5-MIF。