通过等电聚焦分析一种HL - A抗血清。
Analysis of an HL-A antiserum by iso-electric focusing.
作者信息
Reisner E G, Reisner H M, Chung K S, Folds J D, Amos D B
出版信息
J Immunol. 1975 Oct;115(4):1084-6.
An anti-HL-A 3 antiserum with cross-reacting activity for HL-A 1 and HL-A 11 was subjected to isoelectric focusing over a pH 5-8 gradient. The cytotoxic activity of the serum focused into three distinct peaks, one at the basic end of the gradient, one between pH 6 and pH 7 and one at the acidic end. The first and second peaks reacted with HL-A 3 positive cells and HL-A 3 negative cells positive for cross-reacting antigens. The third peak reacted only with HL-A 3 positive cells.
一种对HL - A 1和HL - A 11具有交叉反应活性的抗HL - A 3抗血清，在pH 5 - 8梯度上进行等电聚焦。血清的细胞毒性活性聚焦成三个不同的峰，一个在梯度的碱性端，一个在pH 6和pH 7之间，一个在酸性端。第一个和第二个峰与HL - A 3阳性细胞以及对交叉反应抗原呈阳性的HL - A 3阴性细胞发生反应。第三个峰仅与HL - A 3阳性细胞发生反应。
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