Department of Orthopedics Shanghai First People's Hospital, School of Medicine Shanghai Jiao Tong University, Shanghai, China -
Panminerva Med. 2013 Sep;55(3):283-9.
The goal of this study was to examine the relationship between stearoyl-CoA desaturase 1 (SCD1) and osteogenesis in bone marrow mesenchymal stem cells (BM-MSCs).
SCD1 was overexpressed in BM-MSCs using lentiviral transduction. Then, osteogenesis was induced by low glucose DMEM (10% fetal bovine serum). Alkaline phosphatase (ALP) activity assays, Western blot analyses of SCD1 and osteocalcin, and stainings for ALP and alizarin red were done to assess the extent of osteogenesis in BM-MSCs.
ALP activity was markedly higher in the SCD1 overexpressing cells compared with cells transduced with empty vector or control (untransduced) cells at 1 and 2 weeks after osteogenic induction. By contrast, ALP activities were comparable between control cells and cells transduced with empty vector. Western blot analysis demonstrated that osteocalcin levels were higher in the SCD1 overexpressing cells, compared with cells transduced with empty vector or control cells, at 1 and 2 weeks after osteogenic induction. These findings were corroborated by stainings for ALP and alizarin red that demonstrated a more active osteogenesis in the SCD1 overexpressing cells 2 weeks after osteogenic induction.
Increased levels of SCD1 enhance osteogenesis in BM-MSCs.
本研究旨在探讨硬脂酰辅酶 A 去饱和酶 1（SCD1）与骨髓间充质干细胞（BM-MSCs）成骨之间的关系。
使用慢病毒转导过表达 BM-MSCs 中的 SCD1。然后，在低糖 DMEM（10%胎牛血清）中诱导成骨。通过碱性磷酸酶（ALP）活性测定、SCD1 和骨钙素的 Western blot 分析以及 ALP 和茜素红染色，评估 BM-MSCs 中成骨的程度。
与空载体转导细胞或对照（未转导）细胞相比，在成骨诱导后 1 和 2 周，SCD1 过表达细胞的 ALP 活性明显更高。相比之下，对照细胞和空载体转导细胞的 ALP 活性相当。Western blot 分析表明，在成骨诱导后 1 和 2 周，SCD1 过表达细胞的骨钙素水平高于空载体转导细胞或对照细胞。ALP 和茜素红染色也证实了这一点，即在成骨诱导后 2 周，SCD1 过表达细胞的成骨作用更为活跃。
SCD1 水平的增加可增强 BM-MSCs 的成骨作用。
