Rastogi S C, Clausen J
Immunobiology. 1985 May;169(4):339-45. doi: 10.1016/s0171-2985(85)80015-2.
Electrophoretic studies on microheterogeneity of alpha 2-macroglobulin (alpha 2M) have revealed the presence of an abnormal acidic form of alpha 2M in multiple sclerosis (MS). In the present study we have characterized alpha 2M chemically and compared the inhibitory effect of MS and normal alpha 2M on the mitogen induction of lymphocyte proliferation. The antitrypsin activity and total reducing sugar content of MS alpha 2M were comparable with those of normal alpha 2M. However, the sialic acid content of MS alpha 2M was found to be significantly higher than that of normal alpha 2M. In agreement with previous studies, normal alpha 2M was found to inhibit the mitogen induction of lymphocyte proliferation in more than 90% cell cultures. However, MS alpha 2M was found to inhibit the mitogen-induced lymphocyte proliferation in less than 35 percent cell cultures. The effects of MS or normal alpha 2M on the lymphocyte cultures of either groups were found to be apparently the same. The possible explanations for the loss of the above-mentioned inhibitory effect of MS alpha 2M have been discussed.
对α2-巨球蛋白（α2M）微观异质性的电泳研究表明，在多发性硬化症（MS）中存在一种异常的酸性α2M形式。在本研究中，我们对α2M进行了化学表征，并比较了MS型和正常α2M对丝裂原诱导的淋巴细胞增殖的抑制作用。MS型α2M的抗胰蛋白酶活性和总还原糖含量与正常α2M相当。然而，发现MS型α2M的唾液酸含量明显高于正常α2M。与先前的研究一致，发现正常α2M在超过90%的细胞培养物中抑制丝裂原诱导的淋巴细胞增殖。然而，发现MS型α2M在不到35%的细胞培养物中抑制丝裂原诱导的淋巴细胞增殖。发现MS型或正常α2M对两组淋巴细胞培养物的作用明显相同。已对MS型α2M上述抑制作用丧失的可能原因进行了讨论。
