Suemura M, Kishimoto T
Int Arch Allergy Appl Immunol. 1985;77(1-2):26-31. doi: 10.1159/000233748.
An in vitro experimental system for the induction of human IgE production has been established with human peripheral blood lymphocytes (PBL). For the assessment of IgE produced in vitro, a sensitive ELISA method has been developed by employing monoclonal anti-IgE antibodies. The stimulation of PBL with pokeweed mitogen plus Staphylococcus aureus strain Cowan I induced polyclonal IgE response. T cells from patients with pulmonary tuberculosis, after activation with purified protein derivative and/or IgE, suppressed selectively IgE response, and the suppressive activity was found to be mediated by IgE-specific suppressor factor with affinity for IgE molecules. The suppressive activity was effective both on spontaneous IgE production as well as on mitogen- or antigen-induced IgE response in the PBL culture from atopic patients. The affinity of the suppressor factor for IgE was suggested to be due to the structural similarity with Fc epsilon R on B cells. Absorption experiment suggest that the factor may bear class II major histocompatibility complex (MHC) molecules, although its effect was not MHC-restricted.
已利用人外周血淋巴细胞（PBL）建立了一种用于诱导人IgE产生的体外实验系统。为了评估体外产生的IgE，通过使用单克隆抗IgE抗体开发了一种灵敏的ELISA方法。用商陆有丝分裂原加金黄色葡萄球菌考恩I株刺激PBL可诱导多克隆IgE反应。肺结核患者的T细胞在用纯化蛋白衍生物和/或IgE激活后，选择性地抑制IgE反应，并且发现抑制活性是由对IgE分子具有亲和力的IgE特异性抑制因子介导的。该抑制活性对特应性患者PBL培养物中的自发IgE产生以及有丝分裂原或抗原诱导的IgE反应均有效。抑制因子对IgE的亲和力被认为是由于其与B细胞上的FcεR结构相似。吸收实验表明该因子可能携带II类主要组织相容性复合体（MHC）分子，尽管其作用不受MHC限制。
