Norrby E, Sheshberadaran H, McCullough K C, Carpenter W C, Orvell C
Intervirology. 1985;23(4):228-32. doi: 10.1159/000149609.
Groups of 6-39 monoclonal antibodies identifying 3-18 distinct epitopes on the nucleoprotein (NP), polymerase (P), hemagglutinin (H; equivalent in canine distemper and rinderpest viruses), and fusion (F) components of measles and canine distemper viruses were characterized in immunofluorescence tests with fixed Vero cell cultures infected with measles, canine distemper and rinderpest viruses. The majority of NP-specific monoclonal antibodies reacted with all three viruses, but one-third of the antibodies only reacted with the homologous virus. A few antibodies detected epitopes uniquely shared between either measles and rinderpest viruses or canine distemper and rinderpest viruses. Of the P-specific antibodies, two-thirds only reacted with the homologous virus, one antibody detected an epitope shared between canine distemper and rinderpest viruses, and the rest reacted with all three viruses. Also, the majority of antibodies against the H component were type-specific, but four antibodies reacted both with measles and rinderpest viruses. In contrast, the F component was antigenically highly conserved. 17 of 21 antibodies against this component reacted with all three viruses; one antibody reacted only with measles and rinderpest virus F components, and three antibodies reacted only with the homologous virus. No monoclonal antibody of any specificity selectively reacted with only measles and canine distemper viruses. Furthermore, the measles virus H component appeared to be more closely related to the equivalent rinderpest virus component than to the canine distemper virus component. Thus, it is proposed that rinderpest virus is the archevirus of the morbillivirus group from which canine distemper virus was first to evolve and, more recently (perhaps about 5,000 years ago), measles virus.
在对感染麻疹病毒、犬瘟热病毒和牛瘟病毒的固定Vero细胞培养物进行的免疫荧光试验中，对6至39组单克隆抗体进行了特性分析，这些单克隆抗体可识别麻疹病毒和犬瘟热病毒的核蛋白（NP）、聚合酶（P）、血凝素（H；在犬瘟热病毒和牛瘟病毒中相当于此）以及融合（F）成分上的3至18个不同表位。大多数NP特异性单克隆抗体与所有三种病毒都发生反应，但三分之一的抗体仅与同源病毒发生反应。少数抗体检测到麻疹病毒和牛瘟病毒之间或犬瘟热病毒和牛瘟病毒之间独特共有的表位。在P特异性抗体中，三分之二仅与同源病毒发生反应，一种抗体检测到犬瘟热病毒和牛瘟病毒之间共有的一个表位，其余的与所有三种病毒都发生反应。此外，大多数针对H成分的抗体具有型特异性，但有四种抗体与麻疹病毒和牛瘟病毒都发生反应。相比之下，F成分在抗原性上高度保守。针对该成分的21种抗体中有17种与所有三种病毒都发生反应；一种抗体仅与麻疹病毒和牛瘟病毒的F成分发生反应，三种抗体仅与同源病毒发生反应。没有任何特异性的单克隆抗体仅选择性地与麻疹病毒和犬瘟热病毒发生反应。此外，麻疹病毒的H成分似乎与牛瘟病毒的等效成分比与犬瘟热病毒的成分关系更密切。因此，有人提出牛瘟病毒是麻疹病毒属的原型病毒，犬瘟热病毒首先从该病毒进化而来，最近（可能约5000年前）是麻疹病毒。
