Crawford B J, Vielkind U
In Vitro Cell Dev Biol. 1985 Feb;21(2):79-87. doi: 10.1007/BF02620947.
Distribution and organization of the extracellular glycoproteins, fibronectin and laminin, in clonal cultures of chick retinal pigmented epithelial cells have been investigated using indirect immunofluorescence microscopy. Fibronectin is located on the apical and basal cell surfaces and between the cells in the undifferentiated regions of the colony (outer edge and stratified region). It seems to run parallel to intracellular microfilament bundles and to be associated with them across the cell membrane. In the differentiated region of the colony (center), it is located exclusively on the basal cell surface and seems to be primarily associated with the collagen bundles of the basement membrane. The locations suggest that it may be necessary to permit rapid division and movement of the undifferentiated cells in the outer region of the colony, while stabilizing the sheet of differentiated cells in the colony center. In all regions except the outer edge of the colony, laminin is associated with the basal cell surfaces where it forms a meshwork of short, fine strands. The laminin has a totally different staining pattern from the fibronectin and does not seem to be associated with collagen bundles. The location suggests that laminin may be present in the basal lamina and may be involved in adhesion of the cells to the substratum.
利用间接免疫荧光显微镜技术，对鸡视网膜色素上皮细胞克隆培养物中细胞外糖蛋白纤连蛋白和层粘连蛋白的分布及组织情况进行了研究。纤连蛋白位于细胞顶端和基底表面，以及克隆未分化区域（外缘和分层区域）的细胞之间。它似乎与细胞内微丝束平行，并通过细胞膜与微丝束相关联。在克隆的分化区域（中心），它仅位于细胞基底表面，且似乎主要与基底膜的胶原束相关联。这些位置表明，在克隆外缘的未分化细胞中，可能需要纤连蛋白来促进快速分裂和移动，同时稳定克隆中心的分化细胞片层。在除克隆外缘之外的所有区域，层粘连蛋白都与细胞基底表面相关联，在那里它形成了由短而细的链组成的网络。层粘连蛋白的染色模式与纤连蛋白完全不同，似乎也不与胶原束相关联。这种位置表明，层粘连蛋白可能存在于基底膜中，并可能参与细胞与基质的黏附。
