Crawford B J
Invest Ophthalmol Vis Sci. 1980 Mar;19(3):223-37.
The structure and development of junctional complexes during redifferentiation of chick pigmented epithelial cells in clonal culture have been studied with TEM and colloidal lanthanum. The mature junctional complex consists of a zonula adherens which is usually surmounted by one or more macular gap junctions of varying length. Tight junctions (zonulae occludentes) appear to surround the gap junctions and extend into the zonula adherens. Punctate intermediate junctions appear first. As differentiation progresses, these extend to form fasciae and zonulae adherentes. Focal membrane fustions are found both within and above the developing adherens junctions; gap junctions appear to form adjacent to the latter structures. Colloidal lanthanum passes through the junctional regions between cells in the outer zone of the colony but is stopped by those of the differentiated cells, suggesting that these cells are sealed by fasciae or zonulae occludentes. During redifferentiation, groups of cells undergo slow, coordinated contractions which appear to be involved in developing the differentiated cell shape. These begin shortly after the formation of the junctional complexes and are most active during junctional complex maturation. Once cellular junctional complex differentiation is well established, the contractions cease. The possible roles of the different junctions in the development of cellular shape are discussed.
利用透射电子显微镜（TEM）和胶体镧，对克隆培养的鸡色素上皮细胞再分化过程中连接复合体的结构和发育进行了研究。成熟的连接复合体由一个黏着小带组成，其上方通常有一个或多个长度不等的斑状间隙连接。紧密连接（闭锁小带）似乎围绕着间隙连接并延伸至黏着小带。点状中间连接首先出现。随着分化的进行，这些连接延伸形成筋膜和黏着小带。在发育中的黏着连接内部和上方均发现有局灶性膜融合；间隙连接似乎在后者结构附近形成。胶体镧可穿过克隆体外层区域细胞间的连接区域，但被分化细胞的连接区域阻挡，这表明这些细胞被筋膜或闭锁小带封闭。在再分化过程中，细胞群会进行缓慢、协调的收缩，这似乎与分化细胞形状的形成有关。这些收缩在连接复合体形成后不久开始，在连接复合体成熟期间最为活跃。一旦细胞连接复合体分化充分建立，收缩就会停止。文中讨论了不同连接在细胞形状发育中的可能作用。
