College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
Biosens Bioelectron. 2014 Feb 15;52:391-6. doi: 10.1016/j.bios.2013.09.020. Epub 2013 Sep 15.
An efficient colorimetric biosensor for glucose based on peroxidase-like protein-Fe3O4 and glucose oxidase nanocomposites is reported in this work. Compared with bare MNPs, peroxidase-like casein-MNPs exhibit good catalytic properties, stability, dispersibility. Casein incorporated on MNPs notably improves the affinity toward both H2O2 and TMB, proved by variation in the determined kinetic parameters. As low as 0.2 μM H2O2 can be detected with a linear range from 0.5 μM to 200 μM H2O2. More importantly, the casein/MNP nanocomposite was further used to immobilize GOx and to construct a glucose biosensor for the one-step determination of glucose. This method is simple, inexpensive, highly sensitive, and selective for glucose detection, with a detection limit of 1.0 μM over a linear range from 3 μM to 1000 μM.
本文报道了一种基于过氧化物酶样蛋白-Fe3O4 和葡萄糖氧化酶纳米复合材料的葡萄糖比色生物传感器。与裸 MNPs 相比，过氧化物酶样酪蛋白-MNPs 表现出良好的催化性能、稳定性和分散性。酪蛋白与 MNPs 的结合显著提高了对 H2O2 和 TMB 的亲和力，这可以通过测定动力学参数的变化来证明。低至 0.2 μM 的 H2O2 可以被检测到，线性范围从 0.5 μM 到 200 μM H2O2。更重要的是，酪蛋白/MNP 纳米复合材料进一步用于固定 GOx 并构建葡萄糖生物传感器，用于一步法测定葡萄糖。该方法简单、廉价、对葡萄糖检测具有高灵敏度和选择性，检测限为 1.0 μM，线性范围为 3 μM 至 1000 μM。
