Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
Biosens Bioelectron. 2013 Sep 15;47:502-7. doi: 10.1016/j.bios.2013.03.057. Epub 2013 Mar 30.
C60-carboxyfullerene, C60[C(COOH)2]2, has been proven to function as peroxidase mimetics that can catalyze the reaction of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to produce a blue color reaction. Kinetic studies further indicate that C60[C(COOH)2]2 has an even higher affinity to TMB than that of the natural enzyme, horseradish peroxidase (HRP). C60[C(COOH)2]2/glucose oxidase (GOx)/TMB system provides a novel colorimetric sensor for glucose and shows good response toward glucose detection over a range of 1.0-40μM with a limit of detection 0.5μM (3σ/slope). Moreover, this sensitive and selective sensor can be successfully applied for the quantitative determination of glucose in human serum. The results indicate that it is a simple, cheap, convenient, highly selective, sensitive, and easy handling colorimetric assay.
C60-羧酸富勒烯，C60[C(COOH)2]2，已被证明具有过氧化物酶模拟物的功能，可在 H2O2 存在下催化过氧化物酶底物 3,3',5,5'-四甲基联苯胺（TMB）的反应，产生蓝色反应。动力学研究进一步表明，C60[C(COOH)2]2 对 TMB 的亲和力甚至高于天然酶辣根过氧化物酶（HRP）。C60[C(COOH)2]2/葡萄糖氧化酶（GOx）/TMB 体系为葡萄糖提供了一种新型比色传感器，在 1.0-40μM 范围内对葡萄糖检测具有良好的响应，检测限为 0.5μM（3σ/斜率）。此外，这种灵敏且选择性的传感器可成功用于人血清中葡萄糖的定量测定。结果表明，这是一种简单、廉价、方便、高选择性、灵敏且易于操作的比色分析方法。
