Mount Desert Island Biological Laboratory, PO Box 35, Salisbury Cove, ME 04672, USA; The Graduate School of Biomedical Science and Engineering, The University of Maine, Orono, ME 04469, USA.
Biochem Biophys Res Commun. 2013 Oct 25;440(3):413-8. doi: 10.1016/j.bbrc.2013.09.094. Epub 2013 Oct 1.
Cyclin D genes regulate the cell cycle, growth and differentiation in response to intercellular signaling. While the promoters of vertebrate cyclin D genes have been analyzed, the cis-regulatory sequences across an entire cyclin D locus have not. Doing so would increase understanding of how cyclin D genes respond to the regulatory states established by developmental gene regulatory networks, linking cell cycle and growth control to the ontogenetic program. Therefore, we conducted a cis-regulatory analysis on the cyclin D gene, SpcycD, of the sea urchin, Strongylocentrotus purpuratus, during embryogenesis, identifying upstream and intronic sequences, located within six defined regions bearing one or more cis-regulatory modules each.
细胞周期蛋白 D 基因通过响应细胞间信号来调节细胞周期、生长和分化。虽然已经对脊椎动物细胞周期蛋白 D 基因的启动子进行了分析，但整个细胞周期蛋白 D 基因座的顺式调控序列尚未进行分析。这样做将增加对细胞周期蛋白 D 基因如何响应由发育基因调控网络建立的调控状态的理解，将细胞周期和生长控制与个体发生程序联系起来。因此，我们对海胆 Strongylocentrotus purpuratus 的细胞周期蛋白 D 基因 SpcycD 进行了顺式调控分析，鉴定了位于六个定义区域内的上游和内含子序列，每个区域都带有一个或多个顺式调控模块。
