Weiss J B, Strand M
J Immunol. 1985 Aug;135(2):1421-9.
The chemical and antigenic composition of a major group of concanavalin A-binding glycoprotein antigens of Schistosoma mansoni eggs was examined by the use of monoclonal antibodies. The individual glycoproteins of this group each displayed a very wide range of apparent m.w. and had isoelectric points of less than 5 when analyzed by two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. These glycoproteins could be chemically labeled with 125Iodine by two different methods and biosynthetically labeled with 35S-methionine during in vitro synthesis by isolated eggs. By using five monoclonal antibodies, the individual egg glycoproteins were shown to share several antigenic determinants when analyzed by radioimmunoprecipitation and solid-phase binding assays. These epitopes were present in a high level, and the degree of expression appeared to be developmentally regulated. In addition, Schistosoma haematobium and Schistosoma japonicum eggs contained antigenically related glycoprotein antigens. Preliminary evidence suggests that the epitopes involve carbohydrate moieties.
利用单克隆抗体对曼氏血吸虫卵的一组主要伴刀豆球蛋白A结合糖蛋白抗原的化学和抗原组成进行了研究。通过二维十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析，该组中的各个糖蛋白各自显示出非常广泛的表观分子量范围，并且等电点小于5。这些糖蛋白可以通过两种不同的方法用125碘进行化学标记，并在离体卵的体外合成过程中用35S-甲硫氨酸进行生物合成标记。通过使用五种单克隆抗体，经放射免疫沉淀和固相结合分析表明，各个虫卵糖蛋白具有几种共同的抗原决定簇。这些表位大量存在，并且表达程度似乎受发育调控。此外，埃及血吸虫和日本血吸虫卵含有抗原相关的糖蛋白抗原。初步证据表明，这些表位涉及碳水化合物部分。
