Aronstein W S, Strand M
J Parasitol. 1984 Aug;70(4):545-57.
Gender-specific and pair-dependent glycoprotein antigens of Schistosoma mansoni have been identified. Concanavalin A-binding glycoproteins of unisexually-and bisexually-reared male and female worms were labeled in vitro with 35S-methionine or 125iodine, and the two-dimensional maps of their polypeptides compared. The total protein compositions of male and female worms were very similar. In contrast, the patterns of glycoproteins immunoprecipitated by antisera from mice chronically infected with only male, only female, or both male and female worms were distinctive, indicating that the immune response elicited by the glycoproteins of male and female S. mansoni varied with the type of infection. Radioiodinated cercarial and egg proteins were also studied. Several polypeptides of cercariae and eggs, and 11 of 32 antigenic worm glycoproteins were recognized only by the antiserum from bisexually-infected mice, not by the antisera from unisexually-infected mice. These pair-dependent antigens may be relevant to the immune mechanisms involved in pathogenesis and resistance to reinfection.
已鉴定出曼氏血吸虫的性别特异性和配对依赖性糖蛋白抗原。用35S-甲硫氨酸或125碘在体外标记单性和两性饲养的雄虫和雌虫的伴刀豆球蛋白A结合糖蛋白，并比较其多肽的二维图谱。雄虫和雌虫的总蛋白质组成非常相似。相比之下，仅感染雄虫、仅感染雌虫或同时感染雄虫和雌虫的小鼠抗血清免疫沉淀的糖蛋白模式是独特的，这表明曼氏血吸虫雄虫和雌虫的糖蛋白引发的免疫反应随感染类型而变化。还研究了放射性碘化尾蚴和虫卵蛋白。尾蚴和虫卵的几种多肽以及32种抗原性虫体糖蛋白中的11种仅被两性感染小鼠的抗血清识别，而未被单性感染小鼠的抗血清识别。这些配对依赖性抗原可能与发病机制和抗再感染的免疫机制有关。
