Department of Environmental Technology, Wageningen University, PO Box 17, 6700 EV, Wageningen, The Netherlands,
Appl Microbiol Biotechnol. 2014 Mar;98(6):2751-64. doi: 10.1007/s00253-013-5256-4. Epub 2013 Oct 5.
To improve the coupling of in situ chemical oxidation and in situ bioremediation, a systematic analysis was performed of the effect of chemical oxidation with Fenton's reagent, modified Fenton's reagent, permanganate, or persulfate, on microbial diversity and activity during 8 weeks of incubation in two diesel-contaminated soils (peat and fill). Chemical oxidant and soil type affected the microbial community diversity and biodegradation activity; however, this was only observed following treatment with Fenton's reagent and modified Fenton's reagent, and in the biotic control without oxidation. Differences in the highest overall removal efficiencies of 69 % for peat (biotic control) and 59 % for fill (Fenton's reagent) were partially explained by changes in contaminant soil properties upon oxidation. Molecular analysis of 16S rRNA and alkane monooxygenase (alkB) gene abundances indicated that oxidation with Fenton's reagent and modified Fenton's reagent negatively affected microbial abundance. However, regeneration occurred, and final relative alkB abundances were 1-2 orders of magnitude higher in chemically treated microcosms than in the biotic control. 16S rRNA gene fragment fingerprinting with DGGE and prominent band sequencing illuminated microbial community composition and diversity differences between treatments and identified a variety of phylotypes within Alpha-, Beta-, and Gammaproteobacteria. Understanding microbial community dynamics during coupled chemical oxidation and bioremediation is integral to improved biphasic field application.
为了提高原位化学氧化和原位生物修复的偶联作用,我们系统地分析了芬顿试剂、改良芬顿试剂、高锰酸盐或过硫酸盐原位化学氧化对两种柴油污染土壤(泥炭土和填土)中微生物多样性和活性的影响,这些土壤在 8 周的孵育期内。化学氧化剂和土壤类型影响微生物群落多样性和生物降解活性;然而,这仅在使用芬顿试剂和改良芬顿试剂以及无氧化的生物对照处理后观察到。泥炭土(生物对照)的总体去除效率最高可达 69%,填土(芬顿试剂)的总体去除效率最高可达 59%,这主要是由于氧化作用改变了污染物土壤的性质。16S rRNA 和烷烃单加氧酶(alkB)基因丰度的分子分析表明,芬顿试剂和改良芬顿试剂的氧化作用会对微生物丰度产生负面影响。然而,在化学处理的微宇宙中发生了再生,最终的相对 alkB 丰度比生物对照高 1-2 个数量级。DGGE 的 16S rRNA 基因片段指纹图谱和优势带测序说明了处理之间微生物群落组成和多样性的差异,并鉴定出了α-、β-和γ-变形菌纲中的多种类群。了解化学氧化和生物修复偶联过程中的微生物群落动态是改进两相现场应用的关键。