Department of Microbiology, Institute of Water Research, University of Granada, Spain, C/Ramón y Cajal no 4, 18071 Granada, Spain.
Sci Total Environ. 2013 Feb 15;445-446:347-55. doi: 10.1016/j.scitotenv.2012.12.081. Epub 2013 Jan 23.
The present study focuses on the remediation of diesel-polluted soil using modified Fenton treatment coupled with inorganic NPK fertilizer ("Fenton+NPK"). Studies were carried out in a pilot plant containing 1 m(3) of sandy soil contaminated with 20,000 mg kg(-1) of diesel, placed outdoors at a temperature ranging between 5 and 10 °C. Results showed that NPK-fertilizer as post-treatment stimulated culturable degrading bacteria and enhanced dehydrogenase activity. Fenton+NPK treatment increased total petroleum hydrocarbon (TPH) removal efficacy. Natural attenuation removed 49% of TPH in the surface layer, 23% of TPH in the non-saturated layer and 4% of the TPH in the saturated layer, while the percentage removed of TPH after Fenton+NPK treatment was 58%, 57% and 32% respectively. The results from our study showed that, immediately after soil contamination, occurred a specialization and differentiation of the bacterial community, but after this initial modification, no significant changes of bacterial diversity was observed under natural attenuation conditions. In contrast, when the Fenton's reagent was applied a reduction of the bacterial biodiversity was observed. However, the post-biostimulation did enhance the degrading microbiota and stimulated their degrading biological activity. In conclusion, biostimulation, as a post-treatment step in chemical oxidation, is an effective solution to remediate hydrocarbon-polluted sites.
本研究采用改良的芬顿处理与无机 NPK 肥料（“Fenton+NPK”）相结合，修复受柴油污染的土壤。研究在一个包含 1 立方米受 20000 毫克/千克柴油污染的沙质土壤的中试工厂中进行，该工厂位于室外，温度在 5 到 10°C 之间。结果表明，NPK 肥料作为后处理刺激了可培养的降解菌并增强了脱氢酶活性。Fenton+NPK 处理提高了总石油烃（TPH）的去除效率。自然衰减在表层去除了 49%的 TPH，在非饱和层去除了 23%的 TPH，在饱和层去除了 4%的 TPH，而 Fenton+NPK 处理后去除的 TPH 分别为 58%、57%和 32%。我们的研究结果表明，土壤污染后，细菌群落立即发生专业化和分化，但在这种初始修饰之后，在自然衰减条件下没有观察到细菌多样性的显著变化。相比之下，当应用芬顿试剂时，观察到细菌生物多样性的减少。然而，生物刺激作为化学氧化的后处理步骤，是修复碳氢化合物污染场地的有效解决方案。
