Laboratoire ProBioGEM, Polytech'Lille, Université Lille 1, Avenue Paul Langevin, 59655, Villeneuve d'Ascq, France.
Appl Microbiol Biotechnol. 2013 Dec;97(24):10321-8. doi: 10.1007/s00253-013-5259-1. Epub 2013 Oct 4.
An effective antimicrobial packaging or food contact surface should be able to kill or inhibit micro-organisms that cause food-borne illnesses. Setting up such systems, by nisin adsorption on hydrophilic and hydrophobic surfaces, is still a matter of debate. For this purpose, nisin was adsorbed on two types of low-density polyethylene: the hydrophobic native film and the hydrophilic acrylic acid-treated surface. The antibacterial activity was compared for those two films and it was highly dependent on the nature of the surface and the nisin-adsorbed amount. The hydrophilic surfaces presented higher antibacterial activity and higher amount of nisin than the hydrophobic surfaces. The effectiveness of the activated surfaces was assessed against Listeria innocua and the food pathogens Listeria monocytogenes, Bacillus cereus, and Staphylococcus aureus. S. aureus was more sensitive than the three other test bacteria toward both nisin-functionalized films. Simulation tests to mimic refrigerated temperature showed that the films were effective at 20 and 4 °C with no significant difference between the two temperatures after 30 min of exposure to culture media.
一种有效的抗菌包装或食品接触面应该能够杀死或抑制引起食源性疾病的微生物。通过在亲水性和疏水性表面上吸附乳链菌肽来建立这种系统仍然存在争议。为此，乳链菌肽被吸附在两种类型的低密度聚乙烯上：疏水性的原始膜和亲水性的丙烯酸处理表面。比较了这两种薄膜的抗菌活性，其抗菌活性强烈依赖于表面的性质和吸附的乳链菌肽的量。亲水性表面比疏水性表面具有更高的抗菌活性和更高的乳链菌肽吸附量。对两种激活表面的有效性进行了评估，以测试其对无害李斯特菌和食品病原体李斯特菌、蜡状芽孢杆菌和金黄色葡萄球菌的抑制作用。金黄色葡萄球菌对两种乳链菌肽功能化薄膜的敏感性均高于其他三种测试细菌。模拟冷藏温度的测试表明，在 20 和 4°C 下，两种温度下在接触培养基 30 分钟后没有明显差异，薄膜均有效。
