Laboratoire ProBioGEM, Polytech'Lille, Université Lille 1, Avenue Paul Langevin, 59655, Villeneuve d'Ascq, France.
J Pept Sci. 2013 Jun;19(6):377-85. doi: 10.1002/psc.2512. Epub 2013 Apr 29.
Study of peptides adsorption on surfaces remains a current challenge in literature. A complementary approach, combining X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to investigate the antimicrobial peptide nisin adsorption on hydrophilic and hydrophobic surfaces. The native low density polyethylene was used as hydrophobic support and it was grafted with acrylic acid to render it hydrophilic. XPS permitted to confirm nisin adsorption and to determine its amount on the surfaces. ToF-SIMS permitted to identify the adsorbed bacteriocin type and to observe its distribution and orientation behavior on both types of surfaces. Nisin was more oriented by its hydrophobic side to the hydrophobic substrate and by its hydrophilic side to the outer layers of the adsorbed peptide, in contrast to what was observed on the hydrophilic substrate. A correlation was found between XPS and ToF-SIMS results, the types of interactions on both surfaces and the observed antibacterial activity. Such interfacial studies are crucial for better understanding the peptides interactions and adsorption on surfaces and must be considered when setting up antimicrobial surfaces.
关于表面上的肽吸附的研究仍然是文献中的一个当前挑战。本研究采用了一种结合 X 射线光电子能谱（XPS）和飞行时间二次离子质谱（ToF-SIMS）的互补方法，来研究抗菌肽乳链菌肽在亲水和疏水表面上的吸附。本研究使用原生低密度聚乙烯作为疏水支撑体，并对其进行丙烯酸接枝处理以使其亲水。XPS 允许确认乳链菌肽的吸附，并确定其在表面上的数量。ToF-SIMS 允许鉴定吸附的细菌素类型，并观察其在两种类型的表面上的分布和取向行为。与在亲水基底上观察到的情况相反，乳链菌肽通过其疏水性侧面向疏水基底，通过其亲水性侧面向吸附的肽的外层取向。XPS 和 ToF-SIMS 结果之间存在相关性，这与两种表面上的相互作用类型和观察到的抗菌活性有关。此类界面研究对于更好地理解肽在表面上的相互作用和吸附至关重要，在设置抗菌表面时必须加以考虑。
